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|Antigen||serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies|
|Epitope||AA 300-400, Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SERPINE1 antibody is un-conjugated Alternatives|
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|8 references available|
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Product Details anti-SERPINE1 AntibodyTarget Details SERPINE1 Application Details Handling References for anti-SERPINE1 antibody (ABIN446967) Images
|Specificity||PAI-1 (N315) pAb detects endogenous levels of PAI-1 protein.|
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]|
|Plasmids, Primers & others|
Target Details SERPINE1Product Details anti-SERPINE1 Antibody Application Details Handling References for anti-SERPINE1 antibody (ABIN446967) Images back to top
|Alternative Name||Serpin E1/PAI-1 (SERPINE1 Antibody Abstract)|
|Background||Gene Symbol: SERPINE1|
|Molecular Weight||Theoretical MW: 45 kDa|
|Pathways||p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration|
Application DetailsProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Handling References for anti-SERPINE1 antibody (ABIN446967) Images back to top
|Application Notes||Western Blot 1.0 μg/mL, ELISA, Immunohistochemistry 1:50 - 1:100, Immunocytochemistry/Immunofluorescence 1:50 - 1:100, Immunohistochemistry-Paraffin 1:50 - 1:100, Immunohistochemistry-Frozen 1:50 - 1:100The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in ELISA reported in scientific literature (PMID 28132883).|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Western blot Protocol specific for PAI1/Serpine1 antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry Protocol specific for PAI1/Serpine1 antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details References for anti-SERPINE1 antibody (ABIN446967) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-SERPINE1 antibody (ABIN446967)Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling Images back to top
|Product cited in:||
Gerenu, Martisova, Ferrero, Carracedo, Rantamäki, Ramirez, Gil-Bea: "Modulation of BDNF cleavage by plasminogen-activator inhibitor-1 contributes to Alzheimer's neuropathology and cognitive deficits." in: Biochimica et biophysica acta, Vol. 1863, Issue 4, pp. 991-1001, 2017 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).
Sitaram, Mallikarjuna, Landström, Ljungberg: "Transforming growth factor-β promotes aggressiveness and invasion of clear cell renal cell carcinoma." in: Oncotarget, Vol. 7, Issue 24, pp. 35917-35931, 2016 (Sample species: Human). Further details: Western Blotting
Liao, Wang, Guo, Lin, Chang, Juo: "Let-7g improves multiple endothelial functions through targeting transforming growth factor-beta and SIRT-1 signaling." in: Journal of the American College of Cardiology, Vol. 63, Issue 16, pp. 1685-94, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)
Longchamp, Alonso, Dubuis, Allagnat, Berard, Meda, Saucy, Corpataux, Déglise, Haefliger: "The use of external mesh reinforcement to reduce intimal hyperplasia and preserve the structure of human saphenous veins." in: Biomaterials, Vol. 35, Issue 9, pp. 2588-99, 2014 (Sample species: Human). Further details: Western Blotting
Lee, Oh, Park, Na, Gil, Yang, Lee, Hong: "Urokinase, urokinase receptor, and plasminogen activator inhibitor-1 expression on podocytes in immunoglobulin A glomerulonephritis." in: The Korean journal of internal medicine, Vol. 29, Issue 2, pp. 176-82, 2014 (Sample species: Human).
Berard, Déglise, Alonso, Saucy, Meda, Bordenave, Corpataux, Haefliger: "Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins." in: Journal of vascular surgery, Vol. 57, Issue 5, pp. 1371-82, 2013 (Sample species: Human). Further details: Western Blotting
Dubuis, May, Alonso, Luca, Mylonaki, Meda, Delie, Jordan, Déglise, Corpataux, Saucy, Haefliger: "Atorvastatin-loaded hydrogel affects the smooth muscle cells of human veins." in: The Journal of pharmacology and experimental therapeutics, Vol. 347, Issue 3, pp. 574-81, 2013 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
Cao, Szabolcs, Dutta, Yaqoob, Jagavelu, Wang, Leof, Urrutia, Shah, Mukhopadhyay: "Neuropilin-1 mediates divergent R-Smad signaling and the myofibroblast phenotype." in: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31840-8, 2010 (Sample species: Human). Further details: Western Blotting
ImagesProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling References for anti-SERPINE1 antibody (ABIN446967) back to top