Human CCL18/PARC EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target See all CCL18 products
- CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
- Binding Specificity
- AA 21-89
- Reactivity
- Human
- Detection Method
- Colorimetric
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Host
- Rabbit
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CCL18/PARC in Human samples.
- Brand
- EZ-Set™
- Specificity
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Expression system for standard: E.coli
Immunogen sequence: A21-A89 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Characteristics
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Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
Tissue Specificity: Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. .
- Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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CCL18 (Human) IP-WB Antibody Pair
IP Reactivity: Human IP unconjugated Host: Rabbit
CCL18 (Human) IP-WB Antibody PairIP Reactivity: Human IP unconjugated Host: Rabbit, Mouse
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- Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Restrictions
- For Research Use only
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Expiry Date
- 12 months
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- Target
- CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
- Alternative Name
- CCL18 (CCL18 Products)
- Synonyms
- H7AP1 Matched Antibody Pair, PARC Matched Antibody Pair, RP3-330M21.2 Matched Antibody Pair, AMAC-1 Matched Antibody Pair, AMAC1 Matched Antibody Pair, CKb7 Matched Antibody Pair, DC-CK1 Matched Antibody Pair, DCCK1 Matched Antibody Pair, MIP-4 Matched Antibody Pair, SCYA18 Matched Antibody Pair, cullin 9 Matched Antibody Pair, C-C motif chemokine ligand 18 Matched Antibody Pair, CUL9 Matched Antibody Pair, CCL18 Matched Antibody Pair
- Background
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Protein Function: Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. .
Background: Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60 % identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.
Synonyms: C-C motif chemokine 18,Alternative macrophage activation-associated CC chemokine 1,AMAC-1,CC chemokine PARC,Dendritic cell chemokine 1,DC-CK1,Macrophage inflammatory protein 4,MIP-4,Pulmonary and activation-regulated chemokine,Small-inducible cytokine A18,CCL18(1-68),CCL18(3-69),CCL18(4-69),CCL18,AMAC1, DCCK1, MIP4, PARC, SCYA18,
Full Gene Name: C-C motif chemokine 18
Cellular Localisation: Secreted. - UniProt
- P55774
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