Human Galectin-1 EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target See all LGALS1/Galectin 1 (LGALS1) products
- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
- Binding Specificity
- AA 2-135
- Reactivity
- Human
- Detection Method
- Colorimetric
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Host
- Rabbit
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Galectin-1 in Human samples.
- Brand
- EZ-Set™
- Specificity
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Expression system for standard: E.coli
Immunogen sequence: A2-D135 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Characteristics
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Tissue Specificity: Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. .
- Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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LGALS1 (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
LGALS1 (Human) IP-WB Antibody PairIP Reactivity: Human IP unconjugated Host: Rabbit, Mouse
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- Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Restrictions
- For Research Use only
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Expiry Date
- 12 months
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- Target
- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
- Alternative Name
- LGALS1 (LGALS1 Products)
- Synonyms
- GAL1 Matched Antibody Pair, GBP Matched Antibody Pair, Galectin-1 Matched Antibody Pair, LGAS1 Matched Antibody Pair, LGALS1 Matched Antibody Pair, MGC64502 Matched Antibody Pair, GB10026 Matched Antibody Pair, AA410090 Matched Antibody Pair, Gal-1 Matched Antibody Pair, Galbp Matched Antibody Pair, L-14.5 Matched Antibody Pair, L14 Matched Antibody Pair, Lect14 Matched Antibody Pair, galectin-1 Matched Antibody Pair, Galaptin Matched Antibody Pair, galectin 1 Matched Antibody Pair, galectin-1 Matched Antibody Pair, lectin, galactoside-binding, soluble, 1 L homeolog Matched Antibody Pair, protein enabled Matched Antibody Pair, lectin, galactose binding, soluble 1 Matched Antibody Pair, LGALS1 Matched Antibody Pair, Lgals1 Matched Antibody Pair, CC1G_05003 Matched Antibody Pair, CC1G_05004 Matched Antibody Pair, lgals1.L Matched Antibody Pair, LOC408848 Matched Antibody Pair
- Background
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Protein Function: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. .
Background: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.
Synonyms: Galectin-1,Gal-1,14 kDa laminin-binding protein,HLBP14,14 kDa lectin,Beta-galactoside-binding lectin L-14-I,Galaptin,HBL,HPL,Lactose-binding lectin 1,Lectin galactoside-binding soluble 1,Putative MAPK-activating protein PM12,S-Lac lectin 1,LGALS1,
Full Gene Name: Galectin-1
Cellular Localisation: Secreted, extracellular space, extracellular matrix. - UniProt
- P09382
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