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Human Tryptase/TPSAB1,B2 EZ Set™ ELISA Kit (DIY Antibody Pairs)

ELISA Reactivity: Human ELISA unconjugated AA 1-275 Host: Rabbit
Catalog No. ABIN5510786
  • Target See all TPSB2 products
    TPSB2 (Tryptase beta 2 (TPSB2))
    Binding Specificity
    AA 1-275
    Reactivity
    Human
    Detection Method
    Colorimetric
    Host
    Rabbit
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Tryptase/TPSAB1,B2 in Human samples.
    Brand
    EZ-Set™
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: M1-V275
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Characteristics

    Tissue Specificity: TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin, in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).

    Components
    Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
    Material not included
    1. Microplate reader in standard size.
    2. Automated plate washer.
    3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
    4. Clean tubes and Eppendorf tubes.
    5. Washing buffer (neutral PBS or TBS).
  • Comment

    The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
    The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
    The EZ Set™ Accessory Kit provides:
    Antibody diluent buffer
    ABC diluent buffer
    Sample diluent buffer
    TMB
    TMB stop solution
    5x 96 well plates, plate sealers

    Restrictions
    For Research Use only
  • Buffer
    Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
    Expiry Date
    12 months
  • Target
    TPSB2 (Tryptase beta 2 (TPSB2))
    Alternative Name
    TPSB2 (TPSB2 Products)
    Synonyms
    TPS2 Matched Antibody Pair, tryptaseB Matched Antibody Pair, tryptaseC Matched Antibody Pair, Mcpt6 Matched Antibody Pair, tryptase beta 2 (gene/pseudogene) Matched Antibody Pair, tryptase beta 2 Matched Antibody Pair, TPSB2 Matched Antibody Pair, Tpsb2 Matched Antibody Pair
    Background

    Protein Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. .

    Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles, TPSB2, which represents the beta-II and beta-III tryptase alleles, TPSG1, and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

    Synonyms: Tryptase beta-2,Tryptase-2,3.4.21.59,Tryptase II,TPSB2,TPS2,

    Full Gene Name: Tryptase beta-2

    Cellular Localisation: Secreted. Released from the secretory granules upon mast cell activation.
    UniProt
    P20231
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