Human ADAMTS13 EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target See all ADAMTS13 products
- ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))
- Binding Specificity
- AA 34-688
- Reactivity
- Human
- Detection Method
- Colorimetric
- Host
- Rabbit
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects ADAMTS13 in Human samples.
- Brand
- EZ-Set™
- Specificity
- CHO, Q34-W688
- Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Characteristics
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Tissue Specificity: Plasma. Expressed primarily in liver. .
- Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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- Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Restrictions
- For Research Use only
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Expiry Date
- 12 months
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- Target
- ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))
- Alternative Name
- ADAMTS13 (ADAMTS13 Products)
- Synonyms
- ADAMTS13 Matched Antibody Pair, ADAM-TS13 Matched Antibody Pair, ADAMTS-13 Matched Antibody Pair, C9orf8 Matched Antibody Pair, VWFCP Matched Antibody Pair, vWF-CP Matched Antibody Pair, Gm710 Matched Antibody Pair, ADAM metallopeptidase with thrombospondin type 1 motif 13 Matched Antibody Pair, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 Matched Antibody Pair, ADAM metallopeptidase with thrombospondin type 1 motif, 13 Matched Antibody Pair, ADAMTS13 Matched Antibody Pair, Adamts13 Matched Antibody Pair, adamts13 Matched Antibody Pair
- Background
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Protein Function: Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. .
Background: ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.
Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 13,ADAM-TS 13,ADAM-TS13,ADAMTS-13,3.4.24.87,von Willebrand factor-cleaving protease,vWF-CP,vWF-cleaving protease,ADAMTS13,C9orf8,UNQ6102/PRO20085,
Full Gene Name: A disintegrin and metalloproteinase with thrombospondin motifs 13
Cellular Localisation: Secreted . Secretion enhanced by O-fucosylation of TSP type-1 repeats. - UniProt
- Q76LX8
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