anti-Human Carboxypeptidase E antibody for Western Blotting

Recommended Carboxypeptidase E Antibody (supplied by: Log in to see )

Antigen
Carboxypeptidase E (CPE) Antibodies
  • CPE
  • CPH
  • Cph-1
  • Cph1
  • R74677
  • fat
  • CARBE
  • Cph
  • si:ch211-198g9.2
  • zgc:85981
  • carboxypeptidase E
  • carboxypeptidase E L homeolog
  • CPE
  • cpe
  • Cpe
  • cpe.L
Epitope
Internal Region
13
6
6
5
5
5
4
4
3
3
3
3
3
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
101
58
52
9
8
2
2
2
1
1
1
Host
Rabbit
95
4
3
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
79
39
30
23
13
12
10
9
8
7
5
1
1
1
1
Options
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Catalog No. ABIN2856267
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.558292 ABIN3187709 ELISA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
19.558292 ABIN3184066 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
16.558292 ABIN633904 WB Rabbit N-Term Log in to see Polyclonal 0
12.755681 ABIN5619038 WB Rabbit Center Log in to see Polyclonal 0
12.755681 ABIN5617672 ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.755681 ABIN933894 IP ELISA WB Mouse IgG2a Log in to see 420709-M8100701 0
12.755681 ABIN2138197 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.755681 ABIN6138943 IF WB Rabbit Log in to see Polyclonal 0
10.802612 ABIN6257071 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
10.802612 ABIN1876646 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN4900332 CyTOF FACS ICC IHC IP WB Goat IgG AA 42-453 Log in to see Polyclonal 0
7.8026123 ABIN2787685 IHC WB Rabbit N-Term Log in to see Polyclonal 1
7.8026123 ABIN392011 WB Rabbit Ig Fraction AA 381-412, C-Term Log in to see Polyclonal 1
7.8026123 ABIN1858476 ICC IHC WB Rabbit IgG AA 151-317 Log in to see Polyclonal 0
7.8026123 ABIN1176423 ICC IHC WB Rabbit IgG AA 318-476 Log in to see Polyclonal 0
7.8026123 ABIN2975759 IF/ICC WB Rabbit IgG Log in to see Polyclonal 0
7.8026123 ABIN5963168 IF WB Rabbit IgG Log in to see Polyclonal 0
7.8026123 ABIN2902272 IF/ICC IHC IP WB Rabbit AA 318-476 Log in to see Polyclonal 0
7.8026123 ABIN2902273 IF/ICC IHC IP WB Rabbit AA 151-317 Log in to see Polyclonal 0
7.8026123 ABIN1851022 ELISA WB Rabbit Internal Region Log in to see Polyclonal 0

General

Antigen Carboxypeptidase E (CPE) Antibodies
Epitope Internal Region
(13), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(101), (58), (52), (9), (8), (2), (2), (2), (1), (1), (1)
Host Rabbit
(95), (4), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(79), (39), (30), (23), (13), (12), (10), (9), (8), (7), (5), (1), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Cross-Reactivity Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Xenopus tropicalis
Cross-Reactivity (Details) Pig (95 %), Rat (96 %), Xenopus laevis (83 %), Zebrafish (85 %), Bovine (94 %), Xenopus tropicalis (84 %)
Characteristics Rabbit Polyclonal antibody to Carboxypeptidase E (carboxypeptidase E)
Carboxypeptidase E antibody [N2C2], Internal
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase E. The exact sequence is proprietary.
Isotype IgG

Target Details

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Antigen
Alternative Name Carboxypeptidase E (CPE Antibody Abstract)
Background This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes.

Cellular Localization: Secreted
Molecular Weight 53 kDa
Gene ID 1363
Pathways Peptide Hormone Metabolism, Synaptic Membrane

Application Details

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Application Notes Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*
Comment

Positive Control: Carboxypeptidase H tansfected 293T cell , mouse brain

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.68 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Carboxypeptidase E (CPE) (Internal Region) antibody (ABIN2856267) IHC-P Image Carboxypeptidase E antibody [N2C2], Internal detects Carboxypeptidase E p...
Western Blotting (WB) image for anti-Carboxypeptidase E (CPE) (Internal Region) antibody (ABIN2856267) WB Image Western Blot analysis of CPE expression in transfected 293T cell line by CPE...
Western Blotting (WB) image for anti-Carboxypeptidase E (CPE) (Internal Region) antibody (ABIN2856267) WB Image Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE antibody dil...