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|Antigen||Carboxypeptidase E (CPE) Antibodies|
|Epitope||AA 49-200 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|4 references available|
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|Cross-Reactivity||Rat (Rattus), Mouse (Murine)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human Carboxypeptidase E aa. 49-200|
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|Alternative Name||Carboxypeptidase E (CPE Antibody Abstract)|
|Background||Carboxypeptidase E (CPE), also known as carboxypeptidase H and enkephalin convertase, is found as both a membrane-bound and a soluble glycoprotein in neuroendocrine tissues and adrenal-gland chromaffin granules. The C-terminus forms an amphiphilic alpha-helix, suggesting that this region is responsible for the membrane-bound form. Evidence suggests the active form of CPE is located in the secretory vesicles. CPE appears to have several functions. It is an exopeptidase that cleaves neuropeptides with C-terminal basic amino acids, producing an active form of the peptide. It has also been proposed that membrane-bound CPE is a sorting receptor for regulated secretory pathway (RSP) proteins in the TGN pituitary Golgi and secretory granule membranes. RSP proteins primarily consist of hormones and neuropeptides. Mice that carry a mutation in the CPE gene Cpe[fat] display endocrine disorders such as obesity, infertility, and hyperproinsulinemia. Furthermore, the same endocrine disorders are observed in Cpe[fat] mice where the CPE gene has been effaced by antisense RNA.|
|Molecular Weight||50 kDa|
|Pathways||Peptide Hormone Metabolism, Synaptic Membrane|
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Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
Related Products: ABIN968545
|Restrictions||For Research Use only|
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|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
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|Product cited in:||
Cool, Normant, Shen, Chen, Pannell, Zhang, Loh: "Carboxypeptidase E is a regulated secretory pathway sorting receptor: genetic obliteration leads to endocrine disorders in Cpe(fat) mice." in: Cell, Vol. 88, Issue 1, pp. 73-83, 1997
Shen, Loh: "Intracellular misrouting and abnormal secretion of adrenocorticotropin and growth hormone in cpefat mice associated with a carboxypeptidase E mutation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5314-9, 1997
Varlamov, Fricker: "The C-terminal region of carboxypeptidase E involved in membrane binding is distinct from the region involved with intracellular routing." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6077-83, 1996
Manser, Fernandez, Loo, Goh, Monfries, Hall, Lim: "Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitro." in: The Biochemical journal, Vol. 267, Issue 2, pp. 517-25, 1990
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