anti-Monkey DPP4 antibody for Immunohistochemistry

Recommended DPP4 Antibody (supplied by: Log in to see )

Antigen
Dipeptidyl-Peptidase 4 (DPP4) Antibodies
  • ADABP
  • ADCP2
  • CD26
  • DPPIV
  • TP103
  • MGC81966
  • MOP9.8
  • MOP9_8
  • DPP4
  • si:ch73-2d23.3
  • Cd26
  • Dpp-4
  • THAM
  • dipeptidyl peptidase 4
  • dipeptidyl-peptidase 4 S homeolog
  • prolyl oligopeptidase family protein
  • dipeptidyl-peptidase 4
  • dipeptidylpeptidase 4
  • DPP4
  • dpp4.S
  • AT5G24260
  • dpp4
  • Dpp4
Epitope
Internal Region
50
34
17
14
11
10
10
7
6
6
6
6
5
5
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
527
192
123
28
18
14
8
7
6
5
5
5
4
3
2
2
2
2
1
1
1
Host
Rabbit
429
160
92
23
5
Clonality
Polyclonal
Conjugate
This DPP4 antibody is un-conjugated
62
61
52
32
16
16
15
14
13
13
9
9
9
9
9
8
6
6
5
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
485
188
166
135
110
98
69
38
17
13
13
10
8
6
6
4
4
3
2
1
1
Supplier
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Catalog No. ABIN1048412
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.624921 ABIN960766 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
8.624921 ABIN213568 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
8.624921 ABIN2469485 IHC Rabbit Internal Region Log in to see Polyclonal 0
8.624921 ABIN4306068 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 11D7 0
1 ABIN2620152 IHC IHC (p) WB Rabbit AA 663-712 Log in to see Polyclonal 0
1 ABIN2467512 IHC Rabbit Internal Region Log in to see Polyclonal 0

General

Antigen Dipeptidyl-Peptidase 4 (DPP4) Antibodies
Epitope Internal Region
(50), (34), (17), (14), (11), (10), (10), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(527), (192), (123), (28), (18), (14), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(429), (160), (92), (23), (5)
Clonality
Polyclonal
Conjugate This DPP4 antibody is un-conjugated
(62), (61), (52), (32), (16), (16), (15), (14), (13), (13), (9), (9), (9), (9), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(485), (188), (166), (135), (110), (98), (69), (38), (17), (13), (13), (10), (8), (6), (6), (4), (4), (3), (2), (1), (1)
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Product Details anti-DPP4 Antibody

Target Details DPP4 Application Details Handling Images
Brand IHC-plus™
Specificity Human CD26. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FAP (56 % ).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset (100%) Gorilla, Sheep, Elephant, Bovine, Horse, Pig (94%) Rabbit (88%) Bat, Dog, Cat (81%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human CD26. Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset (100%), Gorilla, Sheep, Elephant, Bovine, Horse, Pig (94%), Rabbit (88%), Bat, Dog, Cat (81%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details DPP4

Product Details anti-DPP4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name DPP4 / CD26 (DPP4 Antibody Abstract)
Background Name/Gene ID: DPP4
Subfamily: Serine S9B
Family: Protease

Synonyms: DPP4, ADCP-2, ADABP, Dipeptidyl peptidase IV, Dipeptidyl-peptidase 4, DPPIV, ADCP2, T-cell activation antigen CD26, CD26, CD26 antigen, Dipeptidyl peptidase 4, Dipeptidylpeptidase 4, DPP IV, TP103
Gene ID 1803
Pathways Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-DPP4 Antibody Target Details DPP4 Handling Images back to top
Application Notes Approved: IHC, IHC-P (1.25 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-DPP4 Antibody Target Details DPP4 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-DPP4 Antibody Target Details DPP4 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Dipeptidyl-Peptidase 4 (DPP4) (Internal Region) antibody (ABIN1048412) Anti-CD26 antibody ABIN1048412 IHC staining of human placenta. Immunohistochemistry ...