anti-Rat (Rattus) Exocyst Complex Component 4 antibody for Immunoprecipitation

Recommended Exocyst Complex Component 4 Antibody (supplied by: Log in to see )

Antigen
Exocyst Complex Component 4 (EXOC4) Antibodies
  • CG2095
  • Dmel\\CG2095
  • Sec8
  • Sec8p
  • dsec8
  • fun
  • SEC8L1
  • DKFZp459N1430
  • SEC8
  • C78892
  • Sec8l1
  • ALR
  • si:ch211-92l17.2
  • si:rp71-18a24.2
  • ATSEC8
  • SUBUNIT OF EXOCYST COMPLEX 8
  • subunit of exocyst complex 8
  • Secretory 8
  • exocyst complex component 4
  • exocyst complex component 4 L homeolog
  • Exocyst complex component 4
  • subunit of exocyst complex 8
  • Sec8
  • EXOC4
  • CpipJ_CPIJ001354
  • Exoc4
  • exoc4
  • exoc4.L
  • sec-8
  • SEC8
Epitope
AA 31-201
6
3
2
2
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
25
16
15
7
7
6
6
5
4
3
3
3
3
3
2
2
2
1
1
Host
Mouse
17
10
Clonality
Monoclonal
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
25
9
8
6
6
6
5
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Catalog No. ABIN968093
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN968092 IF IP IHC WB Mouse IgG2b AA 31-201 Log in to see 14-Sec8 4
1 ABIN364381 IHC (p) IP WB Mouse IgG1 Log in to see 14G1 0
1 ABIN4352428 ICC IF IHC IHC (p) IP WB Mouse IgG1 Log in to see 14G1 0
1 ABIN4352427 ICC IF IHC IHC (p) IP WB Mouse IgG1 Log in to see 14G1 0
1 ABIN5773832 IHC (p) IP WB Mouse IgG1 Log in to see 14G1 0
1 ABIN5697649 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Exocyst Complex Component 4 (EXOC4) Antibodies
Epitope AA 31-201
(6), (3), (2), (2), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(25), (16), (15), (7), (7), (6), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Host Mouse
(17), (10)
Clonality (Clone)
Monoclonal   ( )
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(25), (9), (8), (6), (6), (6), (5)
Pubmed 4 references available
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Product details

Target Details Application Details Handling References Images
Cross-Reactivity Dog (Canine), Mouse (Murine), Chicken, Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat Sec8 aa. 31-201
Clone 14-Sec8
Isotype IgG2b

Target Details

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Antigen
Alternative Name Sec8 (EXOC4 Antibody Abstract)
Background Signal transmission between neurons is regulated by the release of neurotransmitters at the synapse. This process, which is controlled by a complex pathway of membrane trafficking in the presynaptic nerve terminal, leads to membrane fusion and neurotransmitter secretion. Sec8 is a hydrophilic protein of 975 amino acids that is highly expressed in brain and kidney. It is homologus with the yeast secretory protein, Sec8p. In yeast, Sec8 is essential for the Golgi-to-plasma membrane traffic of proteins during constitutive secretion. In rat brain, Sec8 colocalizes with Rab3 and Syntaxin-1a, two important proteins that regulate the fusion of the synaptic vesicle to the plasma membrane. The primary structure of Sec8, a coiled-coil domain (residues 34-99), is commonly found in other proteins important in secretory pathways. This antibody is routinely tested by western blot analysis.
Molecular Weight 110 kDa
Pathways Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis

Application Details

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Comment

Related Products: ABIN968619, ABIN967389

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References

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Product cited in:

Charron, Nakamura, Bacallao, Wandinger-Ness: "Compromised cytoarchitecture and polarized trafficking in autosomal dominant polycystic kidney disease cells." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 111-24, 2000

Duncan, Don-Wauchope, Tapechum, Shipston, Chow, Estibeiro: "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells." in: The Biochemical journal, Vol. 342 Pt 3, pp. 497-501, 1999

Hazuka, Hsu, Scheller: "Characterization of a cDNA encoding a subunit of the rat brain rsec6/8 complex." in: Gene, Vol. 187, Issue 1, pp. 67-73, 1997

Ting, Hazuka, Hsu, Kirk, Bean, Scheller: "rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 21, pp. 9613-7, 1995

Images

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Supplier Images
Western Blotting (WB) image for anti-Exocyst Complex Component 4 (EXOC4) (AA 31-201) antibody (ABIN968093) Western blot analysis of Sec8 on a MDCK cell lysate (Canine kidney, ATCC CCL-34). Lan...
Immunofluorescence (IF) image for anti-Exocyst Complex Component 4 (EXOC4) (AA 31-201) antibody (ABIN968093) Immunofluorescence staining of AN3 CA cells (Human endometrial adenocarcinoma, ATCC H...
 image for anti-Exocyst Complex Component 4 (EXOC4) (AA 31-201) antibody (ABIN968093) anti-Exocyst Complex Component 4 (EXOC4) (AA 31-201) antibody (Image 3)