anti-Human PAX6 antibody for Intracellular Staining

Recommended PAX6 Antibody (supplied by: Log in to see )

Antigen
Paired Box 6 (PAX6) Antibodies
  • pax6
  • an2
  • mgda
  • wagr
  • pax-6
  • pax6a
  • xpax6
  • XLPAX6
  • MGC52531
  • PAX-6
  • AN
  • AN2
  • D11S812E
  • MGDA
  • WAGR
  • Pax-6
  • pax6b
  • 1500038E17Rik
  • AEY11
  • Dey
  • Gsfaey11
  • Sey
  • PAXZF-A
  • Pax6.1
  • cb280
  • fc20e07
  • pax-a
  • pax[zf-a]
  • paxzfa
  • wu:fc20e07
  • zfpax-6a
  • zfpax-6b
  • paired box 6
  • paired box 6 L homeolog
  • paired box 6 S homeolog
  • paired box 6a
  • PAX6
  • pax6
  • Pax6
  • pax6.L
  • pax6.S
  • pax6a
Epitope
AA 406-422
95
93
15
15
11
10
10
8
6
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
283
130
116
54
11
8
7
6
6
5
5
4
4
3
2
2
1
1
1
1
Host
Mouse
151
141
17
2
Clonality
Monoclonal
Conjugate
This PAX6 antibody is un-conjugated
12
12
12
11
9
9
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
BioImaging (BI), Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
226
130
120
117
62
53
44
21
19
13
9
7
4
2
2
1
1
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Catalog No. ABIN2689876
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General

Antigen Paired Box 6 (PAX6) Antibodies
Epitope AA 406-422
(95), (93), (15), (15), (11), (10), (10), (8), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(283), (130), (116), (54), (11), (8), (7), (6), (6), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(151), (141), (17), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAX6 antibody is un-conjugated
(12), (12), (12), (11), (9), (9), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application BioImaging (BI), Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
(226), (130), (120), (117), (62), (53), (44), (21), (19), (13), (9), (7), (4), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-PAX6 Antibody

Target Details PAX6 Application Details Handling References for anti-PAX6 antibody (ABIN2689876) Images
Brand BD Pharmingen™
Characteristics Pax-6 is a member of the paired box (pax) gene family whose protein products are transcription factors involved in development. Pax family members share a highly conserved DNA binding domain that contains six alpha helices (paired domain)and a homeo box domain. Pax-6 has important roles in the development of the eye, nose, central nervous system, and pancreas. Defects in Pax-6 are responsible for various eye malformations including aniridia and Peters anomaly. The O18-1330 monoclonal antibody reacts with human Pax-6. Because the Pax-6 protein sequence is highly conserved among vertebrate species, cross-reactivity with other species is possible. LEFT: Intracellular staining of Pax-6 in neural induction of human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) were cultured in mTeSR® (Stem Cell Technologies) on plates coated with BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277). Embryoid bodies (EB) were made and cultured in medium containing Knockout™ Serum Replacement (Life Technologies) without bFGF for 24 hours and then in medium containing 250 ng/mL human recombinant noggin (R&D Systems) and 10 mM SB 431542 (Tocris) for 4 more days. The EB were then plated on BD Matrigel-coated plates and grown in medium with ITS supplement (Sigma-Aldrich), noggin, and SB 431542. After growth for 21 days, the cells were collected, fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either Purified Mouse anti-Human Pax-6 antibody (solid line histrogram) or Purified Mouse IgG2a, k isotype control (dashed line, Cat. No. 554126). The second-step reagent was PE Goat Anti-Mouse Ig (Multiple Adsorption, Cat. No. 550589). Flow cytometry was performed on a BD LSR™ II flow cytometry system. RIGHT: Immunofluorescent staining of Pax-6 in human embryonic stem cell-derived rosettes. H9 human ES cells (WiCell, Madison, WI) passage 54 cultured in mTeSR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were differentiated towards a neural stem cell lineage. Cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1 % Triton™ X-100 [Perm Buffer III is also suitable], and stained with Purified Mouse anti-Human Pax-6 (pseudo-colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies) and counter-staining was with Hoechst 33342 (Sigma-Aldrich, pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. 561462 Rev. 2 Page 1 of 2

BD Pharmingen™ Purified Mouse anti-Human Pax-6 - Purified - Clone O18-1330 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Pax-6 aa 406-422 Peptide
Clone O18
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details PAX6

Product Details anti-PAX6 Antibody Application Details Handling References for anti-PAX6 antibody (ABIN2689876) Images back to top
Antigen
Alternative Name Pax-6 (PAX6 Antibody Abstract)
Background Synonyms: Oculorhombin, Aniridia type II protein, PAX6, AN2 Entrez Gene ID: 5080
Pathways Peptide Hormone Metabolism, Carbohydrate Homeostasis

Application Details

Product Details anti-PAX6 Antibody Target Details PAX6 Handling References for anti-PAX6 antibody (ABIN2689876) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PAX6 Antibody Target Details PAX6 Application Details References for anti-PAX6 antibody (ABIN2689876) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-PAX6 antibody (ABIN2689876)

Product Details anti-PAX6 Antibody Target Details PAX6 Application Details Handling Images back to top
Product cited in:

Chambers, Fasano, Papapetrou, Tomishima, Sadelain, Studer: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling." in: Nature biotechnology, Vol. 27, Issue 3, pp. 275-80, 2009

Osakada, Jin, Hirami, Ikeda, Danjyo, Watanabe, Sasai, Takahashi: "In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction." in: Journal of cell science, Vol. 122, Issue Pt 17, pp. 3169-79, 2009

Cerf: "Transcription factors regulating beta-cell function." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 155, Issue 5, pp. 671-9, 2006

Glaser, Walton, Maas: "Genomic structure, evolutionary conservation and aniridia mutations in the human PAX6 gene." in: Nature genetics, Vol. 2, Issue 3, pp. 232-9, 1994