anti-Human Proprotein Convertase Subtilisin/kexin Type 2 antibody for ELISA

Recommended Proprotein Convertase Subtilisin/kexin Type 2 Antibody (supplied by: Log in to see )

Antigen
Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) Antibodies
  • NEC 2
  • NEC-2
  • NEC2
  • PC2
  • SPC2
  • PCSK2
  • 6330411F23Rik
  • AI839700
  • Nec-2
  • Nec2
  • Phpp-2
  • pc2
  • nec2
  • spc2
  • pcsk2
  • pcsk2-A
  • proprotein convertase subtilisin/kexin type 2
  • proprotein convertase subtilisin/kexin type 2 L homeolog
  • PCSK2
  • pcsk2
  • Pcsk2
  • pcsk2.L
Epitope
AA 95-107
15
9
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
66
34
28
6
3
3
3
2
2
2
2
2
1
1
Host
Rabbit
60
6
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
49
14
13
13
13
12
7
6
3
3
1
1
Options
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Catalog No. ABIN129594
$ 598.50
Plus shipping costs $45.00 and $22.60 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.232655 ABIN518706 ELISA WB Mouse IgG2a kappa AA 501-609, partial Log in to see 3H4 0
10 ABIN3032332 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 87-116 Log in to see Polyclonal 0
10 ABIN3032333 FACS IHC ELISA WB Rabbit Ig Fraction AA 318-346 Log in to see Polyclonal 0
1 ABIN1828546 ELISA WB Mouse IgG2a, kappa AA 501-609 Log in to see 3H4 0
1 ABIN2342198 ELISA WB Rabbit IgG Center Log in to see Polyclonal 0
1 ABIN2342199 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN2331202 IF IP ELISA WB Rabbit IgG AA 95-107 Log in to see Polyclonal 0
1 ABIN6112282 ELISA HRP Rabbit IgG AA 267-391 Log in to see Polyclonal 0
1 ABIN6112286 ELISA Biotin Rabbit IgG AA 267-391 Log in to see Polyclonal 0
1 ABIN6112284 ELISA FITC Rabbit IgG AA 267-391 Log in to see Polyclonal 0
1 ABIN6115927 ELISA IHC WB Rabbit IgG AA 267-391 Log in to see Polyclonal 0
1 ABIN2331791 ELISA WB Mouse IgG2a kappa AA 501-610 Log in to see 3H4 0
1 ABIN5872485 ELISA WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) Antibodies
Epitope AA 95-107
(15), (9), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(66), (34), (28), (6), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(60), (6), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(49), (14), (13), (13), (13), (12), (7), (6), (3), (3), (1), (1)
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Product details

Target Details Application Details Handling Images
Characteristics Concentration Definition: by UV absorbance at 280 nm
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 95-107 of Human PC2 protein.
Isotype IgG

Target Details

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Antigen
Alternative Name PC2 (PCSK2 Antibody Abstract)
Background PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation.  Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.
Synonyms: E3 SUMO-protein ligase CBX4 Chromobox protein homolog 4 Polycomb 2 homolog Pc2 hPc2
Gene ID 8535
UniProt O00257
Pathways Peptide Hormone Metabolism, cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity

Application Details

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Application Notes This affinity purified antibody has been tested for use in immunofluorescence staining of cultured cells and western blotting using transfected cell lysates.  The antibody is also functional by ELISA.  Reactivity in other immunoassays is unknown.  Dilutions for western blotting represent a starting point dilution and further optimization may be required.  The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure below).  It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1.36 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

Images

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Supplier Images
 image for anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 95-107) antibody (ABIN129594) anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 95-107) antibody
Western Blotting (WB) image for anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 95-107) antibody (ABIN129594) Western blotting. Analysis shows the detection of human PC2 in probed lysates using a...
Immunofluorescence (IF) image for anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 95-107) antibody (ABIN129594) anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS). The ...
 image for anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 95-107) antibody (ABIN129594) Analysis shows the detection of human PC2 in probed lysates using Affinity Purified ...