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|Antigen||Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) Antibodies|
|Epitope||AA 110-125, N-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Purpose||Rabbit IgG polyclonal antibody for Neuroendocrine convertase 2(PCSK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Neuroendocrine convertase 2(PCSK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: proprotein convertase subtilisin/kexin type 2
Protein Name: Neuroendocrine convertase 2
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Proprotein Convertase 2(110-125aa GYRDINEIDINMNDPL), identical to the related mouse and rat sequences.|
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|Alternative Name||PCSK2 (PCSK2 Antibody Abstract)|
PCSK2(Proprotein Convertase, Subtilisin/Kexin-Type, 2), also known as PC2 or NEC2, is an enzyme responsible for the first step in the maturation of many neuroendocrine peptides from their precursors, such as the conversion of proinsulin to insulin intermediates. Seidah et al.(1991) mapped the NEC2 gene to human chromosome 20p11.2-p11.1. Ohagi et al.(1992) observed expression of the chloramphenicol acetyltransferase reporter fusions containing the putative promoter region in insulinoma cells but not in hepatoma cells, consistent with the known tissue-specific pattern of expression of the PC2 gene. Analysis of the level of CAT activity with several deletion mutants identified the region from -1100 to -539 from the translation start site as essential for PC2 promoter activity.
Synonyms: KEX2 like endoprotease 2 antibody|KEX2-like endoprotease 2 antibody|NEC 2 antibody|NEC2 antibody|NEC2_HUMAN antibody|Neuroendocrine convertase 2 antibody|PC2 antibody|PCSK2 antibody|Prohormone convertase 2 antibody|Proprotein convertase 2 antibody|Proprotein convertase subtilisin/kexin type 2 antibody|SPC2 antibody
|Pathways||Peptide Hormone Metabolism, cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity|
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for PCSK2 is approximately 10 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
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