|Antigen||Syntaxin 1A (Brain) (STX1A) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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|Specificity||Please note this product is expected to recognize the products of 2 different genes STX1A (NP_004594.1and NP_001159375.1) and STX1B (NP_443106.1).|
Species reactivity (expected):Human, Rat, Canine, Bovine.
Species reactivity (tested):Mouse.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide with sequence from the N Terminus of the protein sequence according to NP_004594.1, NP_001159375.1, NP_443106.1.|
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|Alternative Name||Syntaxin 1A / STX1A (STX1A Antibody Abstract)|
|Background||Syntaxin is a 35 kDa type II integral membrane protein localized to the plasma membrane at nerve terminals. Syntaxin was identified through its association with synaptotagmin and N-type calcium channel, a subtype of calcium channel on the plasma membrane important for neurotransmitter release. Homologs of syntaxin were identified in various eukaryotes ranging from yeast to human. Evidences for syntaxin's involvement in vesicular transport and neurotransmission include the requirement of its yeast homologs, SSO 1 and 2, in targeting and fusion of Golgi-derived vesicles with the plasma membrane. Syntaxin is also the target of clostridial neurotoxin C1 that block neurotransmitter release. The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF.Synonyms: Neuron-specific antigen HPC-1, STX1, STX1B1, STX1B2, Syntaxin-1A, Syntaxin-1B, Syntaxin-1B1, Syntaxin-1B2|
|Pathways||Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport|
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Peptide ELISA: 1/32000. Western Blot: 0.01 - 0.03 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|