anti-Pig (Porcine) Syntaxin 1A (Brain) antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Syntaxin 1A (Brain) Antibody (supplied by: Log in to see )

Antigen
Syntaxin 1A (Brain) (STX1A) Antibodies
  • HPC-1
  • P35-1
  • STX1
  • SYN1A
  • STX1A
  • hpc-1
  • p35-1
  • stx1
  • sx1a
  • syntaxin1a
  • CG10716
  • CG18615
  • CG31136
  • CG5448
  • CT30033
  • Dm Syx1
  • DmSyx1A
  • Dmel\\CG31136
  • SYX1A
  • Syt1A
  • Syx
  • Syx-1A
  • Syx1
  • Syx1a
  • SyxA
  • anon-EST:Gibbs4
  • anon-WO02059370.54
  • dSyx1
  • l(3)06737
  • synt
  • syt-1A
  • syx
  • syx-1
  • syx-1A
  • syx1
  • syx1A
  • syntaxin 1A
  • syntaxin 1A (brain)
  • Syntaxin 1A
  • syntaxin-1A
  • STX1A
  • Stx1a
  • stx1a
  • Syx1A
  • EDI_005240
  • Tsp_07354
Epitope
N-Term
34
15
13
10
10
8
7
4
4
3
3
2
2
2
2
2
2
1
1
1
1
Reactivity
Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
145
90
77
22
20
18
13
7
4
3
3
2
2
2
1
1
1
1
Host
Rabbit
114
37
7
2
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
131
67
65
24
20
19
16
15
14
7
5
1
1
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Catalog No. ABIN1049369
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1049370 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0

General

Antigen Syntaxin 1A (Brain) (STX1A) Antibodies
Epitope N-Term
(34), (15), (13), (10), (10), (8), (7), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(145), (90), (77), (22), (20), (18), (13), (7), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(114), (37), (7), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(5), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(131), (67), (65), (24), (20), (19), (16), (15), (14), (7), (5), (1), (1)
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Product details

Target Details Application Details Handling Images
Brand IHC-plus™
Specificity Human STX1A / Syntaxin 1A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except STX2 (67 % ).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%) Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%) Elephant (87%) Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).
Purification Immunoaffinity purified
Immunogen Synthetic 15 amino acid peptide from N-Terminus of human STX1A / Syntaxin 1A. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%), Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%), Elephant (87%), Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).

Type of Immunogen: Synthetic peptide

Target Details

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Antigen
Alternative Name STX1A / Syntaxin 1A (STX1A Antibody Abstract)
Background Name/Gene ID: STX1A
Family: Syntaxin/epimorphin

Synonyms: STX1A, Syntaxin-1A, p35-1, SYN1a, Syntaxin 1A, Syntaxin 1A (brain), HPC-1, Neuron-specific antigen HPC-1, STX1
Gene ID 6804
Pathways Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport

Application Details

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Application Notes Approved: IHC, IHC-P (5 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Syntaxin 1A (Brain) (STX1A) (N-Term) antibody (ABIN1049369) Anti-STX1A / Syntaxin 1A antibody ABIN1049369 IHC staining of human brain, cortex. I...