|Antigen||Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) Antibodies|
|Epitope||AA 1-89, N-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This VAMP2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-VAMP2 AntibodyTarget Details VAMP2 Application Details Handling References for anti-VAMP2 antibody (ABIN317061) Images
|Specificity||This antibody recognizes specifically Synaptobrevin 2, but it also shows a low affinity to Synaptobrevin 1. Detects a band of approximately 14 kDa in rat brain lysates.|
|Characteristics||Synonyms: VAMP2, Vesicle-associated membrane protein 2, SYB2|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant Human Synaptobrevin 2 (1-89 aa) purified from E. coli.|
Target Details VAMP2Product Details anti-VAMP2 Antibody Application Details Handling References for anti-VAMP2 antibody (ABIN317061) Images back to top
|Alternative Name||VAMP-2 / Synaptobrevin-2 (VAMP2 Antibody Abstract)|
|Background||Synaptobrevin 2 (Syb2) also known as vesicle associated membrane protein-2 (VAMP2) is a membrane protein that belongs to the v-SNARE family of proteins, and is an essential component in neurotransmitter exocytosis from the presynaptic membrane. It is expressed on the synaptic vesicle, where it is bound by synaptophysin. Synaptophysin targets the vesicle to the presynaptic membrane and releases Syb2, which forms a stable complex with syntaxin and SNAP-25 (both expressed on the target membrane). Nerve cell depolarization and influx of calcium leads to vesicle fusion and neurotransmitter release into the synapse.Synonyms: SYB2, VAMP2, Vesicle-associated membrane protein 2|
|Pathways||Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport|
Application DetailsProduct Details anti-VAMP2 Antibody Target Details VAMP2 Handling References for anti-VAMP2 antibody (ABIN317061) Images back to top
ELISA. Western blot (1/2,000-1/3,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated foruse in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VAMP2 Antibody Target Details VAMP2 Application Details References for anti-VAMP2 antibody (ABIN317061) Images back to top
|Buffer||PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-VAMP2 antibody (ABIN317061)Product Details anti-VAMP2 Antibody Target Details VAMP2 Application Details Handling Images back to top
Pennuto, Bonanomi, Benfenati, Valtorta: "Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles." in: Molecular biology of the cell, Vol. 14, Issue 12, pp. 4909-19, 2003
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