anti-Rat (Rattus) VAMP2 antibody for Enzyme Immunoassay

Recommended VAMP2 Antibody (supplied by: Log in to see )

Antigen
Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) Antibodies
  • zgc:73302
  • wu:fj61c06
  • VAMP2
  • vamp2-a
  • xsybi
  • Syb-2
  • Syb2
  • sybII
  • RATVAMPB
  • RATVAMPIR
  • SYB
  • SYB2
  • VAMP-2
  • syb2
  • vamp2
  • xsybii
  • vesicle-associated membrane protein 2
  • vesicle associated membrane protein 2
  • vesicle associated membrane protein 2 S homeolog
  • vesicle-associated membrane protein 2 (synaptobrevin 2)
  • vesicle associated membrane protein 2 L homeolog
  • vamp2
  • VAMP2
  • vamp2.S
  • Vamp2
  • vamp2.L
Epitope
AA 1-89, N-Term
33
6
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
100
43
40
5
4
3
2
2
2
Host
Mouse
56
44
4
4
3
1
Clonality
Monoclonal
Conjugate
This VAMP2 antibody is un-conjugated
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
98
64
57
26
12
10
9
6
3
2
2
1
Supplier
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Catalog No. ABIN317061
$ 308.00
Plus shipping costs $45.00

General

Antigen Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) Antibodies
Epitope AA 1-89, N-Term
(33), (6), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(100), (43), (40), (5), (4), (3), (2), (2), (2)
Host Mouse
(56), (44), (4), (4), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VAMP2 antibody is un-conjugated
(3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(98), (64), (57), (26), (12), (10), (9), (6), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-VAMP2 Antibody

Target Details VAMP2 Application Details Handling References for anti-VAMP2 antibody (ABIN317061) Images
Specificity This antibody recognizes specifically Synaptobrevin 2, but it also shows a low affinity to Synaptobrevin 1. Detects a band of approximately 14 kDa in rat brain lysates.
Characteristics Synonyms: VAMP2, Vesicle-associated membrane protein 2, SYB2
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Recombinant Human Synaptobrevin 2 (1-89 aa) purified from E. coli.
Clone 3-00E-005
Isotype IgG1
Plasmids, Primers & others

Target Details VAMP2

Product Details anti-VAMP2 Antibody Application Details Handling References for anti-VAMP2 antibody (ABIN317061) Images back to top
Antigen
Alternative Name VAMP-2 / Synaptobrevin-2 (VAMP2 Antibody Abstract)
Background Synaptobrevin 2 (Syb2) also known as vesicle associated membrane protein-2 (VAMP2) is a membrane protein that belongs to the v-SNARE family of proteins, and is an essential component in neurotransmitter exocytosis from the presynaptic membrane. It is expressed on the synaptic vesicle, where it is bound by synaptophysin. Synaptophysin targets the vesicle to the presynaptic membrane and releases Syb2, which forms a stable complex with syntaxin and SNAP-25 (both expressed on the target membrane). Nerve cell depolarization and influx of calcium leads to vesicle fusion and neurotransmitter release into the synapse.Synonyms: SYB2, VAMP2, Vesicle-associated membrane protein 2
Gene ID 6844
UniProt P63027
Pathways Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport

Application Details

Product Details anti-VAMP2 Antibody Target Details VAMP2 Handling References for anti-VAMP2 antibody (ABIN317061) Images back to top
Application Notes ELISA. Western blot (1/2,000-1/3,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated foruse in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-VAMP2 Antibody Target Details VAMP2 Application Details References for anti-VAMP2 antibody (ABIN317061) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-VAMP2 antibody (ABIN317061)

Product Details anti-VAMP2 Antibody Target Details VAMP2 Application Details Handling Images back to top
Background publications

Pennuto, Bonanomi, Benfenati, Valtorta: "Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles." in: Molecular biology of the cell, Vol. 14, Issue 12, pp. 4909-19, 2003

Images

Product Details anti-VAMP2 Antibody Target Details VAMP2 Application Details Handling References for anti-VAMP2 antibody (ABIN317061) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (ABIN317061) anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (ABIN317061) anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (ABIN317061) anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (Image 3)
Western Blotting (WB) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (ABIN317061) anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (Image 4)
Western Blotting (WB) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (ABIN317061) anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibody (Image 5)