anti-Rat (Rattus) VAMP2 antibody for Western Blotting

Recommended VAMP2 Antibody (supplied by: Log in to see )

Antigen
Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) Antibodies
  • zgc:73302
  • wu:fj61c06
  • VAMP2
  • vamp2-a
  • xsybi
  • Syb-2
  • Syb2
  • sybII
  • RATVAMPB
  • RATVAMPIR
  • SYB
  • SYB2
  • VAMP-2
  • syb2
  • vamp2
  • xsybii
  • vesicle-associated membrane protein 2
  • vesicle associated membrane protein 2
  • vesicle associated membrane protein 2 S homeolog
  • vesicle-associated membrane protein 2 (synaptobrevin 2)
  • vesicle associated membrane protein 2 L homeolog
  • vamp2
  • VAMP2
  • vamp2.S
  • Vamp2
  • vamp2.L
Epitope
AA 10-60
30
6
4
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
97
43
39
5
4
3
2
2
2
Host
Rabbit
54
43
4
4
3
1
Clonality
Polyclonal
Conjugate
This VAMP2 antibody is un-conjugated
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
94
61
55
26
11
9
9
6
3
3
2
1
Options
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Catalog No. ABIN351357
$ 454.67
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.819984 ABIN317061 EIA IHC (p) WB Mouse IgG1 AA 1-89, N-Term Log in to see 3-00E-005 1
16.392225 ABIN5663843 IHC WB Rabbit IgG Log in to see Polyclonal 0
15.319985 ABIN1742194 ICC IP IHC WB Guinea Pig AA 2-17 Log in to see Polyclonal 3
12.572241 ABIN351359 IHC WB Sheep Log in to see Polyclonal 7
12.572241 ABIN351358 IHC WB Rabbit IgG AA 10-60 Log in to see Polyclonal 7
12.572241 ABIN351360 IHC WB Sheep IgG Log in to see Polyclonal 7
7.819985 ABIN6265943 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
7.819985 ABIN306417 ELISA WB Mouse kappa Log in to see 3-00E-05 0
4.819985 ABIN1875306 IHC WB Rabbit IgG Log in to see Polyclonal 0
4.819985 ABIN2989601 WB Rabbit IgG Log in to see Polyclonal 0
4.819985 ABIN5966037 WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN960402 WB Rabbit AA 1-50 Log in to see Polyclonal 0
4 ABIN5959943 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN5959944 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN218841 IHC WB Rabbit IgG AA 2-27 Log in to see Polyclonal 0
1 ABIN2745229 IF IP WB Rabbit IgG AA 1-18 Log in to see Polyclonal 0
1 ABIN378207 IHC WB Rabbit IgG AA 10-60 Log in to see Polyclonal 0
1 ABIN5661917 WB Rabbit Log in to see Polyclonal 0
1 ABIN598365 ELISA WB Goat IgG AA 1-94 Log in to see Polyclonal 0
1 ABIN2384221 IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0

General

Antigen Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) Antibodies
Epitope AA 10-60
(30), (6), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus), Mouse (Murine)
(97), (43), (39), (5), (4), (3), (2), (2), (2)
Host Rabbit
(54), (43), (4), (4), (3), (1)
Clonality
Polyclonal
Conjugate This VAMP2 antibody is un-conjugated
(3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(94), (61), (55), (26), (11), (9), (9), (6), (3), (3), (2), (1)
Pubmed 7 references available
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Product Details anti-VAMP2 Antibody

Target Details VAMP2 Application Details Handling References for anti-VAMP2 antibody (ABIN351357) Images
Specificity Specific for VAMP2.
Purification Whole serum
Immunogen A synthetic peptide from aa region 10-60 of human VAMP2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse and xenopus.
Plasmids, Primers & others

Target Details VAMP2

Product Details anti-VAMP2 Antibody Application Details Handling References for anti-VAMP2 antibody (ABIN351357) Images back to top
Antigen
Alternative Name VAMP 2 (VAMP2 Antibody Abstract)
Background The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated proteinSNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.
Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subcellular location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Note: Neuronal synaptic vesicles. Also known as: VAMP-2, Synaptobrevin-2, VAMP2, SYB2.
Pathways Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport

Application Details

Product Details anti-VAMP2 Antibody Target Details VAMP2 Handling References for anti-VAMP2 antibody (ABIN351357) Images back to top
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only

Handling

Product Details anti-VAMP2 Antibody Target Details VAMP2 Application Details References for anti-VAMP2 antibody (ABIN351357) Images back to top
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months

References for anti-VAMP2 antibody (ABIN351357)

Product Details anti-VAMP2 Antibody Target Details VAMP2 Application Details Handling Images back to top
Background publications

Hanson, Stevens: "Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution." in: Nature structural biology, Vol. 7, Issue 8, pp. 687-92, 2000

Martin, Shewan, Millar, Gould, James: "Vesicle-associated membrane protein 2 plays a specific role in the insulin-dependent trafficking of the facilitative glucose transporter GLUT4 in 3T3-L1 adipocytes." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1444-52, 1998

Sutton, Fasshauer, Jahn, Brunger: "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution." in: Nature, Vol. 395, Issue 6700, pp. 347-53, 1998

Jagadish, Fernandez, Hewish, Macaulay, Gough, Grusovin, Verkuylen, Cosgrove, Alafaci, Frenkel, Ward: "Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2." in: The Biochemical journal, Vol. 317 ( Pt 3), pp. 945-54, 1996

Kutay, Ahnert-Hilger, Hartmann, Wiedenmann, Rapoport: "Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane." in: The EMBO journal, Vol. 14, Issue 2, pp. 217-23, 1995

Archer, Ozçelik, Jahn, Francke, Südhof: "Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2." in: The Journal of biological chemistry, Vol. 265, Issue 28, pp. 17267-73, 1990

Elferink, Trimble, Scheller: "Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system." in: The Journal of biological chemistry, Vol. 264, Issue 19, pp. 11061-4, 1989

Images

Product Details anti-VAMP2 Antibody Target Details VAMP2 Application Details Handling References for anti-VAMP2 antibody (ABIN351357) back to top
Supplier Images
 image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 10-60) antibody (ABIN351357) IF on 1 day- old mouse cortical cell culture using Rabbit antibody to Vesicle-associa...
Western Blotting (WB) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 10-60) antibody (ABIN351357) WB on rat brain lysate using Rabbit antibody to Vesicle-associated membrane protein 2...
 image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 10-60) antibody (ABIN351357) IHC on rat brain (cortex) using Rabbit antibody to Vesicle-associated membrane protei...