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|Antigen||Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) Antibodies|
|Epitope||AA 10-60 Alternatives|
|Reactivity||Human, Rat (Rattus), Mouse (Murine) Alternatives|
|Conjugate||This VAMP2 antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-VAMP2 AntibodyTarget Details VAMP2 Application Details Handling References for anti-VAMP2 antibody (ABIN351357) Images
|Specificity||Specific for VAMP2.|
|Immunogen||A synthetic peptide from aa region 10-60 of human VAMP2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse and xenopus.|
|Plasmids, Primers & others|
Target Details VAMP2Product Details anti-VAMP2 Antibody Application Details Handling References for anti-VAMP2 antibody (ABIN351357) Images back to top
|Alternative Name||VAMP 2 (VAMP2 Antibody Abstract)|
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated proteinSNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.
Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subcellular location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Note: Neuronal synaptic vesicles. Also known as: VAMP-2, Synaptobrevin-2, VAMP2, SYB2.
|Pathways||Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport|
Application DetailsProduct Details anti-VAMP2 Antibody Target Details VAMP2 Handling References for anti-VAMP2 antibody (ABIN351357) Images back to top
A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VAMP2 Antibody Target Details VAMP2 Application Details References for anti-VAMP2 antibody (ABIN351357) Images back to top
|Reconstitution||Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.|
|Handling Advice||Avoid freeze and thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Expiry Date||12 months|
References for anti-VAMP2 antibody (ABIN351357)Product Details anti-VAMP2 Antibody Target Details VAMP2 Application Details Handling Images back to top
Hanson, Stevens: "Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution." in: Nature structural biology, Vol. 7, Issue 8, pp. 687-92, 2000
Martin, Shewan, Millar, Gould, James: "Vesicle-associated membrane protein 2 plays a specific role in the insulin-dependent trafficking of the facilitative glucose transporter GLUT4 in 3T3-L1 adipocytes." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1444-52, 1998
Sutton, Fasshauer, Jahn, Brunger: "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution." in: Nature, Vol. 395, Issue 6700, pp. 347-53, 1998
Jagadish, Fernandez, Hewish, Macaulay, Gough, Grusovin, Verkuylen, Cosgrove, Alafaci, Frenkel, Ward: "Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2." in: The Biochemical journal, Vol. 317 ( Pt 3), pp. 945-54, 1996
Kutay, Ahnert-Hilger, Hartmann, Wiedenmann, Rapoport: "Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane." in: The EMBO journal, Vol. 14, Issue 2, pp. 217-23, 1995
Archer, Ozçelik, Jahn, Francke, Südhof: "Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2." in: The Journal of biological chemistry, Vol. 265, Issue 28, pp. 17267-73, 1990
Elferink, Trimble, Scheller: "Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system." in: The Journal of biological chemistry, Vol. 264, Issue 19, pp. 11061-4, 1989
ImagesProduct Details anti-VAMP2 Antibody Target Details VAMP2 Application Details Handling References for anti-VAMP2 antibody (ABIN351357) back to top