Checkpoint Kinase 2 (CHEK2) (N-Term) Peptide
CHEK2
Reactivity: Human
Host: Synthetic
BP, WB
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- Target See all CHEK2 products
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- Protein Region
- N-Term
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Western Blotting (WB)
- Characteristics
- This is a synthetic peptide designed for use in combination with anti-CHEK2 antibody (Catalog #: ARP30335_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
- Purification
- Purified
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
- Concentration
- 1 mg/mL
- Buffer
- Final peptide concentration is 1 mg/mL in PBS.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- Target
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- Synonyms
- CDS1 Peptide, CHK2 Peptide, HuCds1 Peptide, LFS2 Peptide, PP1425 Peptide, RAD53 Peptide, hCds1 Peptide, fa66f08 Peptide, wu:fa66f08 Peptide, zgc:55865 Peptide, Cds1 Peptide, HUCDS1 Peptide, Rad53 Peptide, Chk2 Peptide, cds1 Peptide, chek2 Peptide, chk2 Peptide, hucds1 Peptide, lfs2 Peptide, pp1425 Peptide, rad53 Peptide, checkpoint kinase 2 Peptide, serine/threonine-protein kinase chk2 Peptide, checkpoint kinase 2 L homeolog Peptide, CHEK2 Peptide, chek2 Peptide, Chek2 Peptide, MCYG_07308 Peptide, chek2.L Peptide
- Background
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In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
Protein Interaction Partner: ATM,ATM,AATF,ASF1A,ATM,ATM,ATR,BRCA1,CDC25A,CDC25C,DBF4,E2F1,ERCC6,KPNA2,MDC1,MDM2,MDM4,MRC1,MSH2,MUS81,NBN,PLK1,PLK3,PPP2R2A,PPP2R2B,PPP2R5A,PPP2R5B,PPP2R5C,PPP2R5D,PPP2R5E,RAD9A,TP53,TSSK1B,ATMIN,BRCA1,BRCA2,CDC25A,GINS2,MDC1,MDM2,MSH2,MUS81,PLK1,PLK3,RAD50,TP53BP1,VCP
Protein Size: 586 - Molecular Weight
- 65 kDa
- Gene ID
- 11200
- NCBI Accession
- NM_001005735, NP_001005735
- UniProt
- B7ZBF3
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