anti-Human RFWD2 antibody for Flow Cytometry

Recommended RFWD2 Antibody (supplied by: Log in to see )

Antigen
Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) Antibodies
  • COP1
  • RNF200
  • AI316802
  • C80879
  • Cop1
  • RGD1304773
  • RFWD2
  • cop1
  • rnf200
  • zgc:163067
  • ring finger and WD repeat domain 2
  • ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
  • ring finger and WD repeat domain 2, E3 ubiquitin protein ligase L homeolog
  • RFWD2
  • Rfwd2
  • rfwd2
  • rfwd2.L
Epitope
AA 37-65, N-Term
18
16
9
8
7
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
80
37
7
4
4
3
3
3
2
2
1
1
1
1
Host
Rabbit
76
4
2
Clonality
Polyclonal
Conjugate
This RFWD2 antibody is un-conjugated
3
3
3
3
3
3
Application
Flow Cytometry (FACS), Western Blotting (WB)
80
53
7
5
4
4
1
Options
Supplier
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Available images

Catalog No. ABIN5538462
$ 515.63
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN655275 FACS WB Rabbit Ig Fraction AA 37-65, N-Term Log in to see Polyclonal 0
1 ABIN2237987 FACS ELISA WB Rabbit IgG N-Term, AA 44-73 Log in to see Polyclonal 0
1 ABIN2237976 FACS ELISA WB APC Rabbit IgG N-Term, AA 44-73 Log in to see Polyclonal 0
1 ABIN2237980 FACS ELISA WB FITC Rabbit IgG N-Term, AA 44-73 Log in to see Polyclonal 0
1 ABIN2237985 FACS ELISA WB PE Rabbit IgG N-Term, AA 44-73 Log in to see Polyclonal 0
1 ABIN2237974 FACS ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 44-73 Log in to see Polyclonal 0
1 ABIN2237978 FACS ELISA WB Biotin Rabbit IgG N-Term, AA 44-73 Log in to see Polyclonal 0

General

Antigen Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) Antibodies
Epitope AA 37-65, N-Term
(18), (16), (9), (8), (7), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(80), (37), (7), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(76), (4), (2)
Clonality
Polyclonal
Conjugate This RFWD2 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Flow Cytometry (FACS), Western Blotting (WB)
(80), (53), (7), (5), (4), (4), (1)
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Product Details anti-RFWD2 Antibody

Target Details RFWD2 Application Details Handling Images
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This COP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human COP1.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details RFWD2

Product Details anti-RFWD2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name COP1 (RFWD2 Antibody Abstract)
Background Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.
Molecular Weight 23 kDa
Gene ID 114769
UniProt Q5EG05
Pathways Photoperiodism

Application Details

Product Details anti-RFWD2 Antibody Target Details RFWD2 Handling Images back to top
Application Notes For FACS starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Restrictions For Research Use only

Handling

Product Details anti-RFWD2 Antibody Target Details RFWD2 Application Details Images back to top
Format Liquid
Concentration 0.35 mg/mL
Buffer Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

Product Details anti-RFWD2 Antibody Target Details RFWD2 Application Details Handling back to top
Supplier Images
 image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 37-65), (N-Term) antibody (ABIN5538462) Flow cytometric analysis of A549 cells (right histogram) compared to a negative contr...
 image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 37-65), (N-Term) antibody (ABIN5538462) Western blot analysis in CEM,A549 cell line lysates (35ug/lane).