anti-Chicken AKT antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
C-Term
220
113
106
61
52
49
38
32
30
26
25
21
18
18
18
17
14
13
13
12
12
12
12
12
10
9
9
8
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken
1376
757
607
64
56
55
52
37
31
29
6
6
6
5
4
3
3
3
3
1
1
1
1
Host
Rabbit
1069
362
11
4
2
2
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
55
43
39
39
31
26
12
12
9
7
7
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
1246
665
639
286
208
196
142
106
64
38
32
26
17
11
10
9
6
4
3
3
2
1
1
1
1
1
1
1
Options
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Pubmed
2 references available

Available images

Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783)
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783)
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783)
Catalog No. ABIN94783
$ 637.50
Plus shipping costs $45.00 and $22.60 dry ice

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope C-Term
(220), (113), (106), (61), (52), (49), (38), (32), (30), (26), (25), (21), (18), (18), (18), (17), (14), (13), (13), (12), (12), (12), (12), (12), (10), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Chicken
(1376), (757), (607), (64), (56), (55), (52), (37), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(1069), (362), (11), (4), (2), (2)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(55), (43), (39), (39), (31), (26), (12), (12), (9), (7), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(1246), (665), (639), (286), (208), (196), (142), (106), (64), (38), (32), (26), (17), (11), (10), (9), (6), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN94783) Images
Specificity AKT Antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Characteristics AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Sterility Sterile filtered
Immunogen AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type: Peptide
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN94783) Images back to top
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN94783) Images back to top
Application Notes AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Comment

Gene Name: AKT1
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN94783) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-AKT antibody (ABIN94783)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Matsuda, Takano, Kageyama, Hatakeyama, Shakunaga, Kitajima, Yamazaki, Hattori: "Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock." in: Cardiovascular research, Vol. 76, Issue 1, pp. 132-40, 2007

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN94783) back to top
Supplier Images
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783) Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardi...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783) Western Blot of Rabbit AKT Antibody. Lane 1: NIR MW protein ladder. Lane 2: AKT1, rec...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783) Western Blot of Rabbit Anti-AKT antibody. Lane 1: NIH/3T3 whole cell lysate. Load: 20...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783) Western Blot of simultaneous detection of unphosphorylated and phosphorylated Rabbit ...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN94783) Immunohistochemistry of Rabbit Anti-AKT antibody. Tissue: (A) normal colon tissue, (B...