anti-Chimpanzee AKT antibody for Immunoprecipitation

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
C-Term
216
112
107
58
57
49
37
32
30
26
25
21
19
18
18
18
14
13
13
12
12
12
12
12
10
10
9
9
8
7
7
6
6
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chimpanzee
1305
744
601
69
55
54
52
44
31
29
6
6
6
5
4
3
3
3
2
1
1
1
1
Host
Mouse
1033
355
11
4
2
2
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
55
43
39
39
31
26
12
12
7
7
7
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1209
620
599
283
205
194
133
110
61
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN349616
$ 389.00
Plus shipping costs $45.00 and $23.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1043730 IP IHC ELISA WB Mouse IgG1 kappa C-Term Log in to see 18F10-E5 4
1 ABIN5550837 EIA IF IHC (p) IP WB Mouse IgG1 Ser473 Log in to see 17F6-B11 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope C-Term
(216), (112), (107), (58), (57), (49), (37), (32), (30), (26), (25), (21), (19), (18), (18), (18), (14), (13), (13), (12), (12), (12), (12), (12), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Chimpanzee
(1305), (744), (601), (69), (55), (54), (52), (44), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(1033), (355), (11), (4), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(55), (43), (39), (39), (31), (26), (12), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1209), (620), (599), (283), (205), (194), (133), (110), (61), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Specificity Anti-AKT monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Chimpanzee
Characteristics AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Purification purified
Sterility Sterile filtered
Immunogen Anti-AKT monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of of human AKT1 protein.
Immunogen Type: Peptide
Clone 18F10-E5
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes Anti-AKT Antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Comment

Gene Name: AKT1

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is three (3) months from date of opening.
Expiry Date 3 months

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN349616) Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. L...
ELISA image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN349616) ELISA of Mouse Anti-AKT Antibody. Antigen: HCT-116 cell line. Coating amount: Conflue...
SDS-PAGE (SDS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN349616) SDS PAGE of Mouse anti-AKT Monoclonal Antibody. Lane 1: Reduced Mouse anti-AKT Monocl...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN349616) Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. L...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN349616) Western Blot of Mouse anti-AKT Monoclonal Antibody. Lane 1: His-AKT1 Recombinant Prot...