anti-Cow (Bovine) AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
N-Term
216
112
108
58
56
48
37
32
30
26
25
21
19
18
18
18
14
13
13
12
12
12
12
12
10
10
9
9
8
7
7
6
6
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
1305
745
602
69
55
53
52
43
30
28
6
6
6
4
4
3
3
3
3
1
1
1
1
Host
Rabbit
1031
357
11
4
2
2
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
55
43
39
39
31
26
12
12
7
7
7
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
1209
622
601
283
205
195
134
110
61
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2792186
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.579589 ABIN4900954 WB Mouse C-Term Log in to see 0
12.579589 ABIN926917 WB Rabbit N-Term Log in to see Polyclonal 0
10.8156 ABIN2705430 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
10.8156 ABIN2705426 IC IF IHC WB Rabbit pTyr315 Log in to see Polyclonal 0
10.8156 ABIN2704459 IC IF IHC WB Rabbit C-Term, pSer473 Log in to see Polyclonal 0
10.8156 ABIN2972331 IF/ICC IHC WB KLH Rabbit IgG C-Term, pSer473 Log in to see Polyclonal 0
10.8156 ABIN2972655 IF/ICC IHC WB KLH Rabbit IgG Center, pSer246 Log in to see Polyclonal 0
10 ABIN214085 IHC IHC (p) WB Rabbit IgG AA 121-170 Log in to see Polyclonal 0
9.3156 ABIN223011 IP IHC WB Rabbit IgG Log in to see Polyclonal 2
7.8156 ABIN2705427 IHC WB Rabbit Center Log in to see Polyclonal 0
7.8156 ABIN2705425 IC IF IP WB Rabbit pSer308 Log in to see Polyclonal 0
7.8156 ABIN2705428 IP IHC WB Rabbit Center Log in to see Polyclonal 0
7.8156 ABIN2705429 IHC WB Rabbit Center Log in to see Polyclonal 0
7.8156 ABIN2704463 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
7.8156 ABIN2704462 IHC WB Rabbit C-Term Log in to see Polyclonal 0
7.8156 ABIN2704465 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
7.8156 ABIN2704461 IHC WB Rabbit C-Term, pSer473 Log in to see Polyclonal 0
7.8156 ABIN2972757 IHC WB KLH Rabbit IgG C-Term, pThr450 Log in to see Polyclonal 0
7.8156 ABIN2972623 IHC WB Rabbit Center Log in to see Polyclonal 0
7.8156 ABIN2972759 IHC WB Rabbit N-Term Log in to see Polyclonal 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope N-Term
(216), (112), (108), (58), (56), (48), (37), (32), (30), (26), (25), (21), (19), (18), (18), (18), (14), (13), (13), (12), (12), (12), (12), (12), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(1305), (745), (602), (69), (55), (53), (52), (43), (30), (28), (6), (6), (6), (4), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(1031), (357), (11), (4), (2), (2)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(55), (43), (39), (39), (31), (26), (12), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Western Blotting (WB)
(1209), (622), (601), (283), (205), (195), (134), (110), (61), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN2792186) Images
Sequence RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK
Predicted Reactivity Cow: 86%, Dog: 100%, Goat: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rat: 100%, Sheep: 86%
Characteristics This is a rabbit polyclonal antibody against AKT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human AKT1
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN2792186) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Alias Symbols: AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA
Protein Size: 480
Molecular Weight 56 kDa
Gene ID 207
NCBI Accession NM_005163, NP_005154
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN2792186) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 480 AA

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN2792186) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AKT antibody (ABIN2792186)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Kim, Kim, Lee: "Expression of caveolin-1 is correlated with Akt-1 in colorectal cancer tissues." in: Experimental and molecular pathology, Vol. 80, Issue 2, pp. 165-70, 2006

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN2792186) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (N-Term) antibody (ABIN2792186) WB Suggested Anti-AKT1 Antibody Positive Control: Lane 1: 50ug preterm baboon mus...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (N-Term) antibody (ABIN2792186) Lanes: 1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laev...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (N-Term) antibody (ABIN2792186) Rabbit Anti-AKT1 Antibody AV Formalin Fixed Paraffin Embedded Tissue: Human Lung Tis...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (N-Term) antibody (ABIN2792186) WB Suggested Anti-AKT1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Posi...