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|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AKT antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling Images
|Specificity||Clone 11E6 specifically recognizes PKB phosphorylated at serine 473.|
|Characteristics||Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase|
|Purification||Subsequent Ultrafiltration and Size Exclusion Chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic phosphopeptide conjugated to KLH. Epitope: phosphoserine 473 P Q F pS Y S ARemarks: Molecular weight of antigen: 60 kDa|
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling Images back to top
|Alternative Name||AKT1 / PKB (AKT1 Antibody Abstract)|
|Background||The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far, the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway. Activated PKB is phosphorylated at serine 473 (mTOR/Rictor or DNA-PK) and threonine 308 (PDK1).Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase|
|Research Area||Signaling, Protein Modifications, Kinases/Phosphatases|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling Images back to top
ELISA: Use at 0.05 μg/mL. Immunoblotting (Western): 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Provided Positive Control: Cell lysate from pervanadate treated HepG2 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Positive Control: Cell lysate from HepG2 pervanadate treatedFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min. with pervanadateReconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °Cup to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freeze / thaw cycles.|
|Expiry Date||12 months|
ImagesProduct Details anti-AKT Antibody Target Details AKT Application Details Handling back to top