anti-Human AKT antibody for Cytometry by Time of Flight

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • beta-actin
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • actin beta
  • CG4006 gene product from transcript CG4006-RE
  • Actin 5C
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • Act5C
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
AA 2-480
223
133
113
67
55
51
40
36
22
22
21
20
18
18
16
13
12
12
11
11
10
10
10
9
9
9
8
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1258
669
504
63
58
57
53
46
33
30
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Mouse
939
383
12
4
2
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
56
43
41
40
34
26
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
1094
559
480
255
186
173
129
85
67
33
26
15
13
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN4899445
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4899444 CyTOF FACS ICC SimWes WB Mouse IgG2b AA 2-480, pan Log in to see 281046 4
1 ABIN4900619 CyTOF FACS IHC SimWes WB Rabbit IgG pSer473 Log in to see Polyclonal 12
1 ABIN4278979 CyTOF FACS ICC IF WB Mouse IgG2 Log in to see MM0647-2L45

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 2-480
(223), (133), (113), (67), (55), (51), (40), (36), (22), (22), (21), (20), (18), (18), (16), (13), (12), (12), (11), (11), (10), (10), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1258), (669), (504), (63), (58), (57), (53), (46), (33), (30), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(939), (383), (12), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(56), (43), (41), (40), (34), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
(1094), (559), (480), (255), (186), (173), (129), (85), (67), (33), (26), (15), (13), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN4899445) Images
Specificity Detects human Akt1 in direct ELISAs. Reactivity with Akt1 from other species has not been determined. In direct ELISAs, no cross-reactivity with recombinant human (rh) Akt2 or rhAkt3 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt1, Ser2-Ala480, Accession # P31749
Clone 302407
Isotype IgG1

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN4899445) Images back to top
Antigen
Alternative Name Akt1 (AKT1 Antibody Abstract)
Background Alternative Names: AKT, EC 2.7.11, EC 2.7.11.1, PKB alpha, PKBMGC99656, PRKBA, Protein kinase B, Proto-oncogene c-Akt, rac protein kinase alpha, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, RACPKB-ALPHA, v-akt murine thymoma viral oncogene homolog 1
Gene ID 207
UniProt P31749
Research Area Kinases/Phosphatases, Cell Cycle, Apoptosis/Necrosis, Cancer
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN4899445) Images back to top
Application Notes Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN4899445) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-AKT antibody (ABIN4899445)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Xu, Gao, Fan, Meng, Goldberg, Abounader, Ressom, Laterra, Rosen: "Effect of Akt inhibition on scatter factor-regulated gene expression in DU-145 human prostate cancer cells." in: Oncogene, Vol. 26, Issue 20, pp. 2925-38, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN4899445) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 2-480) antibody (ABIN4899445) Detection of Akt1 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast canc...