anti-Human AKT antibody for Immunoprecipitation

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
AA 1-481
214
112
108
58
57
49
37
32
29
26
25
21
19
18
18
18
14
13
13
12
12
12
12
12
10
10
9
8
8
7
7
6
6
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1302
741
601
69
55
54
52
44
31
29
6
6
6
5
4
3
3
3
3
1
1
1
1
Host
Mouse
1031
355
11
4
2
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
55
43
39
38
30
26
12
12
7
7
7
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
1208
620
599
282
205
192
133
110
61
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN393423
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.640009 ABIN559829 IF IHC (p) IP ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 2E11 0
23.640009 ABIN559827 IP PLA RNAi ELISA Mouse IgG2a kappa AA 381-480, partial Log in to see 4C3 0
1 ABIN6259867 ELISA ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal 19
1 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Log in to see 18F3-H11 4
1 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN1043730 IP IHC ELISA WB Mouse IgG1 kappa C-Term Log in to see 18F10-E5 4
1 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 4
1 ABIN94783 FM IHC (fp) IP ELISA WB Rabbit C-Term Log in to see Polyclonal 2
1 ABIN223011 IP IHC WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN964561 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11 0
1 ABIN349616 IP IHC ELISA WB Mouse IgG1 kappa C-Term Log in to see 18F10-E5 0
1 ABIN5556658 EIA IF IHC (fro) IHC (p) IP WB Rabbit AA 460-480, C-Term Log in to see Polyclonal 0
1 ABIN349626 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 0
1 ABIN223018 IP WB Rabbit IgG pSer473 Log in to see Polyclonal 6
1 ABIN2705425 IC IF IP WB Rabbit pSer308 Log in to see Polyclonal 0
1 ABIN2705428 IP IHC WB Rabbit Center Log in to see Polyclonal 0
1 ABIN2704463 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2704465 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN615022 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN5550837 EIA IF IHC (p) IP WB Mouse IgG1 Ser473 Log in to see 17F6-B11 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 1-481
(214), (112), (108), (58), (57), (49), (37), (32), (29), (26), (25), (21), (19), (18), (18), (18), (14), (13), (13), (12), (12), (12), (12), (12), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1302), (741), (601), (69), (55), (54), (52), (44), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Mouse
(1031), (355), (11), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(55), (43), (39), (38), (30), (26), (12), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(1208), (620), (599), (282), (205), (192), (133), (110), (61), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN393423) Images
Specificity AKT1 (AAH00479, 1 a.a. ~ 481 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: AKT1 (AAH00479.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 2E11
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN393423) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background

Other Names: RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC

Gene Description: v-akt murine thymoma viral oncogene homolog 1

Gene Summary: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq

Molecular Weight 55686 DA
Gene ID 207
NCBI Accession NP_001014431, NP_001014432, NP_005154
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN393423) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN393423) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-AKT antibody (ABIN393423)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Izquierdo, Truan, Mengual, Mallofré, Alcaraz: "HER-2/AKT expression in upper urinary tract urothelial carcinoma: prognostic implications." in: Anticancer research, Vol. 30, Issue 6, pp. 2439-45, 2010

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Lin, Lin, Yu: "miR-149* induces apoptosis by inhibiting Akt1 and E2F1 in human cancer cells." in: Molecular carcinogenesis, Vol. 49, Issue 8, pp. 719-27, 2010

Slattery, Herrick, Lundgreen, Fitzpatrick, Curtin, Wolff: "Genetic variation in a metabolic signaling pathway and colon and rectal cancer risk: mTOR, PTEN, STK11, RPKAA1, PRKAG2, TSC1, TSC2, PI3K and Akt1." in: Carcinogenesis, Vol. 31, Issue 9, pp. 1604-11, 2010

Benesch, Schneider, Voelker, Kapp, Caffier, Krockenberger, Dietl, Kammerer, Schmidt: "The clinicopathological and prognostic relevance of pyruvate kinase M2 and pAkt expression in breast cancer." in: Anticancer research, Vol. 30, Issue 5, pp. 1689-94, 2010

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN393423) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (Image 2)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) AKT1 monoclonal antibody (M03), clone 2E11 Western Blot analysis of AKT1 expression i...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monocl...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded h...
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentrati...
Immunoprecipitation (IP) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) Immunoprecipitation of AKT1 transfected lysate using anti-AKT1 monoclonal antibody an...
ELISA image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-481) antibody (ABIN393423) Detection limit for recombinant GST tagged AKT1 is approximately 3ng/ml as a capture ...