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|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies|
|Epitope||AA 2-480, pan Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AKT antibody is un-conjugated Alternatives|
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling References for anti-AKT antibody (ABIN4899444) Images
|Specificity||Detects human, mouse and rat Akt in direct ELISAs and Western blots.|
|Purification||Protein A or G purified from hybridoma culture supernatant|
|Sterility||0.2 μm filtered|
|Immunogen||E. coli-derived recombinant human Akt1, Ser2-Ala480, Accession # P31749|
|Plasmids, Primers & others|
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN4899444) Images back to top
|Alternative Name||Akt (AKT1 Antibody Abstract)|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN4899444) Images back to top
Western Blot 0.2 μg/mL
Simple Western 2 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN4899444) Images back to top
|Reconstitution||Reconstitute at 0.5 mg/mL in sterile PBS.|
|Buffer||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Storage||4 °C,-20 °C,-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-AKT antibody (ABIN4899444)Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
|Product cited in:||
Tolvanen, Dash, Polianskyte-Prause, Dumont, Lehtonen: "Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes." in: Journal of cell science, Vol. 128, Issue 24, pp. 4588-600, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).
Bifulco, Longanesi-Cattani, Franco, Pavone, Mugione, Di Carluccio, Masucci, Arra, Pirozzi, Stoppelli, Carriero: "Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion." in: PLoS ONE, Vol. 7, Issue 9, pp. e44806, 2012 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).
Deng, Han, Yan, Tian, Tao, Kang, Li: "Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 4, pp. 463-73, 2010 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).
Han, Wu, Deng, Tao, Guo, Tian, Kang, Zhang, Yan: "Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1225-35, 2010 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).
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