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|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies|
|Epitope||AA 71-184 Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AKT antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
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Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling References for anti-AKT antibody (ABIN968206) Images
|Cross-Reactivity||Dog (Canine), Rat (Rattus), Mouse (Murine)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human PKBalpha/Akt a.a. 71-184|
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN968206) Images back to top
|Alternative Name||Akt (AKT1 Antibody Abstract)|
Rac (Related to the A and C kinases) protein kinase shows 73% and 68% to Protein Kinase C and PKA, respectively, and is also known as Protein Kinase B (PKB). It was also identified as the product of the v-akt oncogene from T-cell lymphomas. PKB mRNA is expressed in a wide variety of cell lines and has been detected in porcine brain, heart, liver, kidney, muscle, and ovary. Rac phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. PKB is activated in response to growth factors through the activation of PI3-kinase and Ras. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of PKB coincident with its phosphorylation and activation. Cellular stress leading to the activation of the p38 MAP kinase also induces PKB activation, indicating a multiplicity of signaling pathways that activate PKB. Upon activation, PKB associates with some members of the PKC family of kinases, such as PKCdelta and PKCzeta. Downstream of PKB, GSK3 is thus far the only identified substrate for PKB phosphorylated in vivo.
|Molecular Weight||59 kDa|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN968206) Images back to top
Related Products: ABIN968551, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN968206) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-AKT antibody (ABIN968206)Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
|Product cited in:||
Pekarsky, Koval, Hallas, Bichi, Tresini, Malstrom, Russo, Tsichlis, Croce: "Tcl1 enhances Akt kinase activity and mediates its nuclear translocation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3028-33, 2000
Nakatani, Thompson, Barthel, Sakaue, Liu, Weigel, Roth: "Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21528-32, 1999
Frech, Andjelkovic, Ingley, Reddy, Falck, Hemmings: "High affinity binding of inositol phosphates and phosphoinositides to the pleckstrin homology domain of RAC/protein kinase B and their influence on kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8474-81, 1997
Cross, Alessi, Cohen, Andjelkovich, Hemmings: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, 1996
Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991
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