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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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37.63551 | ABIN1043753 | IP IHC ELISA WB | Biotin | Mouse | IgG1 kappa | pThr308, Internal Region | Log in to see | 18F3-H11 | 4 | |
35.49702 | ABIN6255359 | ELISA ICC IF IHC WB | Rabbit | IgG | pSer473 | Log in to see | Polyclonal | 19 | ||
33.13551 | ABIN964561 | IP IHC ELISA WB | Mouse | IgG1 kappa | pSer473 | Log in to see | 17F6-B11 | 0 | ||
30.13849 | ABIN5542263 | ELISA FACS ICC IHC WB | Mouse | IgG1 | AA 1-150 | Log in to see | 1F7E10 | 0 | ||
29.5 | ABIN6259867 | ELISA ICC IF IHC IP WB | Rabbit | IgG | Log in to see | Polyclonal | 19 | |||
29.497019 | ABIN4900619 | CyTOF FACS IHC SimWes WB | Rabbit | IgG | pSer473 | Log in to see | Polyclonal | 19 | ||
27.13849 | ABIN349616 | IP IHC ELISA WB | Mouse | IgG1 kappa | C-Term | Log in to see | 18F10-E5 | 0 | ||
27.13849 | ABIN3183239 | ELISA IHC WB | Rabbit | IgG | N-Term | Log in to see | Polyclonal | 0 | ||
25.63551 | ABIN1043729 | IP IHC ELISA WB | Mouse | IgG1 kappa | pThr308 | Log in to see | 18F3-H11 | 4 | ||
24.13849 | ABIN5596663 | ELISA FACS FM IHC WB | Biotin | Mouse | IgG2a | Internal Region | Log in to see | 14E5-A2-B2-H9 | 0 | |
24.13849 | ABIN5596665 | ELISA FACS FM IHC WB | FITC | Mouse | IgG2a | Internal Region | Log in to see | 14E5-A2-B2-H9 | 0 | |
24.13849 | ABIN5596664 | ELISA FACS FM IHC WB | Biotin | Mouse | IgG2a | Internal Region | Log in to see | 5E5-F5-D7 | 0 | |
24.13849 | ABIN5596666 | ELISA FACS FM IHC WB | FITC | Mouse | IgG2a | Internal Region | Log in to see | 5E5-F5-D7 | 0 | |
24.13849 | ABIN3181909 | ELISA IHC WB | Rabbit | IgG | pThr450 | Log in to see | Polyclonal | 0 | ||
24.13849 | ABIN3183235 | ELISA IHC WB | Rabbit | IgG | Internal Region | Log in to see | Polyclonal | 0 | ||
22.63849 | ABIN3023505 | IHC WB | Rabbit | IgG | pThr450 | Log in to see | Polyclonal | 1 | ||
21.997019 | ABIN393423 | IF IHC IP WB | Mouse | IgG2a kappa | AA 1-481 | Log in to see | 2E11 | 5 | ||
21.997019 | ABIN6256119 | ELISA ICC IF IHC WB | Rabbit | IgG | pSer124 | Log in to see | Polyclonal | 0 | ||
21.997019 | ABIN6256564 | ELISA ICC IF IHC WB | Rabbit | IgG | pThr450 | Log in to see | Polyclonal | 0 | ||
21.997019 | ABIN4902878 | IF IHC WB | Rabbit | IgG | Log in to see | Polyclonal | 0 |
General |
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Antigen | V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies |
Epitope | pSer473, Internal Region Alternatives |
Reactivity | Human, Mouse (Murine), Rat (Rattus), Monkey Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This AKT antibody is conjugated to Biotin Alternatives |
Application |
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Alternatives
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4 references available |
Supplier | Log in to see |
Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling References for anti-AKT antibody (ABIN1043752) Images |
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Specificity | Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined. |
Characteristics | AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research. |
Immunogen |
Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein. Immunogen Type: Peptide |
Clone | 17F6-B11 |
Isotype | IgG1 kappa |
Plasmids, Primers & others | |
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN1043752) Images back to top |
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Antigen | |
Alternative Name | Akt (AKT1 Antibody Abstract) |
Background |
AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research. Synonyms: phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, phospho AKT antibody, Anti-AKT pS473 Antibody Biotin Conjugated |
Gene ID | 207, 62241011 |
UniProt | P31749 |
Pathways | PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling |
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN1043752) Images back to top |
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Application Notes | Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. |
Comment |
Gene Name: AKT1 |
Restrictions | For Research Use only |
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN1043752) Images back to top |
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Format | Lyophilized |
Reconstitution | Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL |
Concentration | 1.0 mg/mL |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C/-20 °C |
Storage Comment | Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
Expiry Date | 12 months |
References for anti-AKT antibody (ABIN1043752)Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top |
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Product cited in: |
Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001 Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991 Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987 |
ImagesProduct Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN1043752) back to top |
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