anti-Mouse (Murine) AKT antibody for Immunohistochemistry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
pSer473
235
117
114
59
59
50
38
32
29
27
25
21
20
19
18
18
18
14
13
13
13
12
12
12
12
10
10
10
9
8
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1367
798
639
72
56
55
54
44
31
29
6
6
6
5
4
3
3
3
3
1
1
1
1
Host
Rabbit
1063
393
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
56
43
41
40
33
26
15
15
10
10
8
6
6
6
6
6
6
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
1262
654
601
289
229
206
137
110
61
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN6255359
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.457311 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
8.457311 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Log in to see 18F3-H11 4
8.457311 ABIN4900619 CyTOF FACS IHC SimWes WB Rabbit IgG pSer473 Log in to see Polyclonal 16
8.457311 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 4
8.457311 ABIN6255660 ELISA ICC IF IHC WB Rabbit IgG pThr308 Log in to see Polyclonal 5
8.457311 ABIN393423 IF IHC IP WB Mouse IgG2a kappa AA 1-481 Log in to see 2E11 5
8.457311 ABIN6255360 ELISA ICC IF IHC WB Rabbit IgG pSer473 Log in to see Polyclonal 4
8.457311 ABIN964561 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11 0
8.457311 ABIN349626 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 0
8.457311 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
8.457311 ABIN1981392 FACS IF IHC WB Rabbit IgG AA 428-457, C-Term Log in to see Polyclonal 0
8.457311 ABIN2707319 IHC WB Rabbit N-Term Log in to see Polyclonal 0
8.457311 ABIN2705427 IHC WB Rabbit Center Log in to see Polyclonal 0
8.457311 ABIN2705430 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
8.457311 ABIN2705426 IC IF IHC WB Rabbit pTyr315 Log in to see Polyclonal 0
8.457311 ABIN2705428 IP IHC WB Rabbit Center Log in to see Polyclonal 0
8.457311 ABIN2705429 IHC WB Rabbit Center Log in to see Polyclonal 0
8.457311 ABIN2704463 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
8.457311 ABIN2704460 IHC WB Rabbit C-Term, pTyr474 Log in to see Polyclonal 0
8.457311 ABIN2704462 IHC WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473
(235), (117), (114), (59), (59), (50), (38), (32), (29), (27), (25), (21), (20), (19), (18), (18), (18), (14), (13), (13), (13), (12), (12), (12), (12), (10), (10), (10), (9), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1367), (798), (639), (72), (56), (55), (54), (44), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(1063), (393), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(56), (43), (41), (40), (33), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(1262), (654), (601), (289), (229), (206), (137), (110), (61), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 19 references available
Supplier Log in to see

Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN6255359) Images
Specificity Phospho-Akt(Ser473) Antibody detects endogenous levels of Akt only when phosphorylated at Sersine 473
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human Akt around the phosphorylation site of Serin 473
Isotype IgG
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN6255359) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background

Description: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI3P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.

Gene: AKT1

Molecular Weight 60kDa
Gene ID 207
UniProt P31749, P31751, Q9Y243
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN6255359) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN6255359) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-AKT antibody (ABIN6255359)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Xie, Cao, Yang, Xu, Wei, Wang: "Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3K/Akt Signaling Pathway In Vitro." in: BioMed research international, Vol. 2017, pp. 2869405, 2018

Ning, Liang, Liu, Wang, Edwards, Xia, Polin, Dyson, Taub, Mohammad, Azmi, Zhao, Ge: "Targeting ERK enhances the cytotoxic effect of the novel PI3K and mTOR dual inhibitor VS-5584 in preclinical models of pancreatic cancer." in: Oncotarget, Vol. 8, Issue 27, pp. 44295-44311, 2018

Wang, Li, Li, Pan, Bai, Huang: "Angelicin inhibits liver cancer growth in vitro and in vivo." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 5441-5449, 2018

Yang, Liu, Jin, Tao, Yang: "Estrogen receptor β exhibited anti-tumor effects on osteosarcoma cells by regulating integrin, IAP, NF-kB/BCL-2 and PI3K/Akt signal pathway." in: Journal of bone oncology, Vol. 9, pp. 15-20, 2018

Su, Li, Ma, Zhao, Liu, Wang, Edwards, Taub, Lin, Ge: "Targeting PI3K, mTOR, ERK, and Bcl-2 signaling network shows superior antileukemic activity against AML ex vivo." in: Biochemical pharmacology, Vol. 148, pp. 13-26, 2018

Geng, Zhu, Wang, Xie, Li, Wu, Li, Chen, Wang, Meng, Zhang, Chen, Zhong: "P53 modulates hepatic insulin sensitivity through NF-κB and p38/ERK MAPK pathways." in: Biochemical and biophysical research communications, Vol. 495, Issue 3, pp. 2139-2144, 2018

Huang, Zhang, Hou, Wang, Liu, Zhang, Chen, Zhu: "LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 194, 2018

Wei, An, Jin, Li, Xu: "Inhibition of insulin resistance by PGE1 via autophagy-dependent FGF21 pathway in diabetic nephropathy." in: Scientific reports, Vol. 8, Issue 1, pp. 9, 2018

Chai, Bai, Li, Chen, Zhang: "Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1076-1080, 2018

Liu, Geng, Zhang, Wang, Zhang, Duan, Zhang: "Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway." in: Marine drugs, Vol. 16, Issue 3, 2018

Liu, Tang, Liu, Zhang, He, Li, Gao, Tang, Jin, Kong: "Increased autophagy in EOC re-ascites cells can inhibit cell death and promote drug resistance." in: Cell death & disease, Vol. 9, Issue 4, pp. 419, 2018

Li, Feng, Wei, Liu, Yang, Hou, Zhao, Zhang, Yang: "The Aqueous Extract of Gynura divaricata (L.) DC. Improves Glucose and Lipid Metabolism and Ameliorates Type 2 Diabetes Mellitus." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 8686297, 2018

Li, Zhao, Lin, Gong, An: "TREM2 inhibits inflammatory responses in mouse microglia by suppressing the PI3K/NF-κB signaling." in: Cell biology international, 2018

Huang, Song, Tao, Shao, Zeng, Xu, Qi, Sun: "Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells." in: PLoS ONE, Vol. 13, Issue 4, pp. e0195610, 2018

Wang, Li, Chen, Yang: "Umbilical cord‑derived mesenchymal stem cells can inhibit the biological functions of melanoma A375 cells." in: Oncology reports, Vol. 40, Issue 1, pp. 511-517, 2018

Ge, Liang, Li, An, Ren, Yue, Wu: "DcR3 induces proliferation, migration, invasion, and EMT in gastric cancer cells via the PI3K/AKT/GSK-3β/β-catenin signaling pathway." in: OncoTargets and therapy, Vol. 11, pp. 4177-4187, 2018

Liu, Zheng, Zhang, Wang, Yang, Bai, Dai: "Fucoxanthin Activates Apoptosis via Inhibition of PI3K/Akt/mTOR Pathway and Suppresses Invasion and Migration by Restriction of p38-MMP-2/9 Pathway in Human Glioblastoma Cells." in: Neurochemical research, Vol. 41, Issue 10, pp. 2728-2751, 2017

He, Cao, Liu, Li, Xu, Liu, Shi: "Quercetin reverses experimental pulmonary arterial hypertension by modulating the TrkA pathway." in: Experimental cell research, Vol. 339, Issue 1, pp. 122-34, 2016

Yao, Jiang, Zhang, Liu, Du, Feng: "Chrysin alleviates allergic inflammation and airway remodeling in a murine model of chronic asthma." in: International immunopharmacology, Vol. 32, pp. 24-31, 2016

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN6255359) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN6255359) Western blot analysis on 293 cell lysate using Phospho-Akt(Ser473) Antibody.The lane ...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN6255359) ABIN6266448 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue ...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN6255359) Phospho-Akt(Ser473) Antibody for IHC in human brain tissue
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN6255359) ABIN6266448 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue ...