anti-Mouse (Murine) AKT antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
pSer473
213
112
108
58
57
49
37
32
29
26
25
21
19
18
18
18
14
13
13
12
12
12
12
12
10
10
9
9
8
7
7
6
6
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
1302
741
601
68
55
54
52
44
31
29
6
6
6
5
4
3
3
3
3
1
1
1
1
Host
Mouse
1031
355
11
4
2
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
55
43
39
38
30
26
12
12
7
7
7
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1208
619
599
282
204
191
134
109
61
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4278999
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.952263 ABIN4279000 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 0
9.952263 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
9.952263 ABIN615022 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
9.952263 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 4
9.952263 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6 1
9.952263 ABIN214085 IHC IHC (p) WB Rabbit IgG AA 121-170 Log in to see Polyclonal 0
9.952263 ABIN615269 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
9.952263 ABIN213755 ELISA ICC IF IHC IHC (p) WB Rabbit pSer473 Log in to see Polyclonal 0
9.952263 ABIN213759 ELISA IHC IHC (p) WB Rabbit pThr308 Log in to see Polyclonal 0
9.952263 ABIN5945070 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see ST48-09 0
9.952263 ABIN4278998 ELISA IHC IHC (p) IP WB Mouse IgG1 Log in to see 3A3 0
9.952263 ABIN213898 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
9.952263 ABIN5945071 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see ST509 0
9.952263 ABIN5945074 ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see JJ08-46 0
9.952263 ABIN498818 IHC (p) Rabbit pTyr326 Log in to see Polyclonal 0
9.952263 ABIN498224 IHC (p) Rabbit Log in to see Polyclonal 0
9.952263 ABIN5945075 ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see SY28-05 0
9.952263 ABIN5557411 IF (cc) IHC (p) WB Rabbit IgG pSer473 Log in to see 12A1 0
9.952263 ABIN5945076 IHC IHC (p) IP WB Rabbit IgG Log in to see SD08-12 0
1 ABIN725195 IF (p) IHC (p) WB Rabbit IgG AA 445-480 Log in to see Polyclonal 4

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473
(213), (112), (108), (58), (57), (49), (37), (32), (29), (26), (25), (21), (19), (18), (18), (18), (14), (13), (13), (12), (12), (12), (12), (12), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(1302), (741), (601), (68), (55), (54), (52), (44), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Mouse
(1031), (355), (11), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(55), (43), (39), (38), (30), (26), (12), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1208), (619), (599), (282), (204), (191), (134), (109), (61), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Purification Protein A purified
Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.
Clone 17F6-B11
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name Akt1 (AKT1 Antibody Abstract)
Background Gene Symbol: AKT1
Molecular Weight Theoretical MW: 56 kDa
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes Western Blot 1:500-1:3000, Flow Cytometry, ELISA 1:20000, Immunohistochemistry 20 μg/mL, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Flow Cytometry: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Western Blot: AKT1 Antibody (17F6.B11) - Lane 1: non-phosphorylated AKT in untreate...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Western Blot: AKT1 Antibody (17F6.B11) - A Lane 1) PDGF stimulated NIH 3T3 cells 10...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Western Blot: AKT1 Antibody (17F6.B11) - Lane 1: A431 cells. Lane 2: A431 cells sti...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunohistochemistry-Paraffin: AKT1 Antibody (17F6.B11) - Analysis of Tissue: human...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Western Blot: AKT1 Antibody (17F6.B11) - Analysis using the Biotin conjugate of ABI...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunohistochemistry-Paraffin: AKT1 Antibody (17F6.B11) - Analysis of Biotin conjug...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Immunohistochemistry-Paraffin: AKT1 Antibody (17F6.B11) - Analysis using the Biotin...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4278999) Western Blot: AKT1 Antibody (17F6.B11) - Lane 1: unstimulated NIH/3T3 lysates conta...