anti-Rabbit AKT antibody for Fluorescence Microscopy

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Reactivity
Human, Mouse (Murine), Rabbit, Rat (Rattus)
1367
798
639
72
56
55
54
44
31
29
6
6
5
5
4
3
3
3
3
1
1
1
1
Host
Rabbit
1063
393
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is conjugated to Biotin
55
43
41
40
33
26
15
15
10
10
8
6
6
6
6
6
6
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
1262
654
601
290
229
205
136
111
61
38
32
26
11
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN5596660
$ 389.00
Plus shipping costs $45.00

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Reactivity Human, Mouse (Murine), Rabbit, Rat (Rattus)
(1367), (798), (639), (72), (56), (55), (54), (44), (31), (29), (6), (6), (5), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(1063), (393), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is conjugated to Biotin
(55), (43), (41), (40), (33), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(1262), (654), (601), (290), (229), (205), (136), (111), (61), (38), (32), (26), (11), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Cross-Reactivity (Details) Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
Purification AKT Antibody reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography.
Immunogen

Immunogen: Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.

Immunogen Type: Native Protein

Isotype IgG
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background

Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1 Antibody, AKT2 Antibody, Akt3 Antibody, Biotin, BAC

Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Gene Name: AKT1, AKT2, AKT3

Gene ID 10000
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Biotin Conjugated AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:500 - 1:2,000

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months