anti-Rat (Rattus) AKT antibody for Immunofluorescence

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • Actin 5C
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • Act5C
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
AA 71-184
224
133
114
65
56
51
40
36
22
22
21
21
18
18
16
13
12
12
11
11
10
10
10
9
9
9
8
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
1264
674
508
63
58
57
53
45
33
30
8
8
6
5
5
3
3
3
2
2
1
1
1
1
Host
Mouse
944
384
12
4
2
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
56
43
41
40
34
26
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1114
575
495
261
193
172
128
86
72
33
26
15
13
11
11
4
4
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN968206
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.738697 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
11.738697 ABIN2705430 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
11.738697 ABIN2704459 IC IF IHC WB Rabbit C-Term, pSer473 Log in to see Polyclonal 0
11.738697 ABIN2705426 IC IF IHC WB Rabbit pTyr315 Log in to see Polyclonal 0
11.738697 ABIN2705425 IC IF IP WB Rabbit pSer308 Log in to see Polyclonal 0
11.738697 ABIN968218 IF IP IHC WB Mouse IgG1, kappa Log in to see 55-PKBa-Akt 5
11.738697 ABIN615022 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
11.738697 ABIN264497 IF WB Rabbit IgG pSer473 Log in to see 14-06-14 3
11.738697 ABIN1532436 IF IHC ELISA WB Rabbit IgG AA 95-144 Log in to see Polyclonal 1
11.738697 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6 1
11.738697 ABIN1531419 IF IHC ELISA WB Rabbit IgG AA 212-261, pSer246 Log in to see Polyclonal 0
11.738697 ABIN1532536 IF IHC ELISA WB Rabbit IgG AA 292-341 Log in to see Polyclonal 0
11.738697 ABIN968205 IF IP IHC WB Mouse IgG1 AA 71-184 Log in to see 7-Akt-PKBalpha 5
11.738697 ABIN1869972 IF IHC WB Rabbit IgG pThr308 Log in to see Polyclonal 0
11.738697 ABIN1869963 IF IHC WB Rabbit IgG pThr450 Log in to see Polyclonal 0
11.738697 ABIN1869970 IF IHC WB Rabbit IgG pThr450 Log in to see Polyclonal 0
11.738697 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11 0
11.738697 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
11.738697 ABIN2455515 FACS IF IHC WB Mouse IgG1 Log in to see 1G5 0
11.738697 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 71-184
(224), (133), (114), (65), (56), (51), (40), (36), (22), (22), (21), (21), (18), (18), (16), (13), (12), (12), (11), (11), (10), (10), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(1264), (674), (508), (63), (58), (57), (53), (45), (33), (30), (8), (8), (6), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(944), (384), (12), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(56), (43), (41), (40), (34), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1114), (575), (495), (261), (193), (172), (128), (86), (72), (33), (26), (15), (13), (11), (11), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN968206) Images
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PKBalpha/Akt a.a. 71-184
Clone 7-Akt-PKBalpha
Isotype IgG1

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN968206) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background Rac (Related to the A and C kinases) protein kinase shows 73% and 68% to Protein Kinase C and PKA, respectively, and is also known as Protein Kinase B (PKB). It was also identified as the product of the v-akt oncogene from T-cell lymphomas. PKB mRNA is expressed in a wide variety of cell lines and has been detected in porcine brain, heart, liver, kidney, muscle, and ovary. Rac phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. PKB is activated in response to growth factors through the activation of PI3-kinase and Ras. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of PKB coincident with its phosphorylation and activation. Cellular stress leading to the activation of the p38 MAP kinase also induces PKB activation, indicating a multiplicity of signaling pathways that activate PKB. Upon activation, PKB associates with some members of the PKC family of kinases, such as PKCdelta and PKCzeta. Downstream of PKB, GSK3 is thus far the only identified substrate for PKB phosphorylated in vivo.
Synonyms: PKBalpha
Molecular Weight 59 kDa
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN968206) Images back to top
Comment

Related Products: ABIN968551, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN968206) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-AKT antibody (ABIN968206)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Pekarsky, Koval, Hallas, Bichi, Tresini, Malstrom, Russo, Tsichlis, Croce: "Tcl1 enhances Akt kinase activity and mediates its nuclear translocation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3028-33, 2000

Nakatani, Thompson, Barthel, Sakaue, Liu, Weigel, Roth: "Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21528-32, 1999

Frech, Andjelkovic, Ingley, Reddy, Falck, Hemmings: "High affinity binding of inositol phosphates and phosphoinositides to the pleckstrin homology domain of RAC/protein kinase B and their influence on kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8474-81, 1997

Cross, Alessi, Cohen, Andjelkovich, Hemmings: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, 1996

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN968206) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 71-184) antibody (ABIN968206) Western blot analysis of Akt on HCT-8 cell lysate (Human colorectal adenocarcinoma, A...
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 71-184) antibody (ABIN968206) Immunofluorescent staining of WI-38 cells with Anti-Akt.
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 71-184) antibody (ABIN968206) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 71-184) antibody (Image 3)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 71-184) antibody (ABIN968206) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 71-184) antibody (Image 4)