anti-Rat (Rattus) AKT antibody for Immunofluorescence

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
pSer473
229
115
113
58
58
51
36
33
29
25
23
22
21
19
18
18
18
14
13
13
11
11
11
11
10
10
10
9
9
8
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1328
750
590
64
56
56
54
37
31
28
7
6
6
5
4
3
3
3
1
1
1
1
1
Host
Rabbit
1024
389
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
56
43
43
40
33
26
15
15
10
10
8
6
6
6
6
6
6
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1217
620
595
265
201
200
136
88
64
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN153477
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.099852 ABIN2705425 IC IF IP WB Rabbit pSer308 Log in to see Polyclonal 0
12.099852 ABIN2705430 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
12.099852 ABIN2705426 IC IF IHC WB Rabbit pTyr315 Log in to see Polyclonal 0
12.099852 ABIN2704459 IC IF IHC WB Rabbit C-Term, pSer473 Log in to see Polyclonal 0
12.099852 ABIN615022 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
12.099852 ABIN2715840 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
12.099852 ABIN1532436 IF IHC ELISA WB Rabbit IgG AA 95-144 Log in to see Polyclonal 1
12.099852 ABIN264497 IF WB Rabbit IgG pSer473 Log in to see 14-06-14 3
12.099852 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6 1
12.099852 ABIN1531419 IF IHC ELISA WB Rabbit IgG AA 212-261, pSer246 Log in to see Polyclonal 0
12.099852 ABIN1532536 IF IHC ELISA WB Rabbit IgG AA 292-341 Log in to see Polyclonal 0
12.099852 ABIN1869963 IF IHC WB Rabbit IgG pThr450 Log in to see Polyclonal 0
12.099852 ABIN1869970 IF IHC WB Rabbit IgG pThr450 Log in to see Polyclonal 0
12.099852 ABIN1869972 IF IHC WB Rabbit IgG pThr308 Log in to see Polyclonal 0
12.099852 ABIN2715843 FACS IF IHC WB Mouse IgG1 Log in to see 1G5 0
12.099852 ABIN2715841 FACS IF IHC WB Mouse IgG1 Log in to see 4C11 0
12.099852 ABIN2715838 FACS IF IHC WB Mouse IgG1 Log in to see 4G4 0
12.099852 ABIN2715836 FACS IF IHC WB Mouse IgG1 Log in to see 4D6 0
12.099852 ABIN2715837 FACS IF IHC WB Mouse IgG1 Log in to see 3E3 0
12.099852 ABIN2715839 FACS IF IHC WB Mouse IgG1 Log in to see 3B4 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473
(229), (115), (113), (58), (58), (51), (36), (33), (29), (25), (23), (22), (21), (19), (18), (18), (18), (14), (13), (13), (11), (11), (11), (11), (10), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1328), (750), (590), (64), (56), (56), (54), (37), (31), (28), (7), (6), (6), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Host Rabbit
(1024), (389), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(56), (43), (43), (40), (33), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1217), (620), (595), (265), (201), (200), (136), (88), (64), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN153477) Images
Specificity AKT [Ser473]
Purification Immunogen affinity purified
Immunogen Synthetic peptide: CHFPQFSYSAS conjugated to KLH, corresponding to amino acids 460-480 of Human AKT.
Isotype IgG
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN153477) Images back to top
Antigen
Alternative Name Akt1 (AKT1 Antibody Abstract)
Background Gene Symbol: AKT1
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN153477) Images back to top
Application Notes Western Blot 1:200-1:1000, Flow Cytometry 1:1:10 - 1:1000, ELISA 1:15000-1:60000, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:40, Immunohistochemistry-Paraffin 1:10-1:500For immunohistochemistry no pre-treatment of sample is required.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN153477) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-AKT antibody (ABIN153477)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Jung, Jung, Yim, Jung, Choi, Kang, Park, Oh, Kim, Lee, Chung: "Predictive microRNAs for lymph node metastasis in endoscopically resectable submucosal colorectal cancer." in: Oncotarget, Vol. 7, Issue 22, pp. 32902-15, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Cuesto, Jordán-Álvarez, Enriquez-Barreto, Ferrús, Morales, Acebes: "GSK3β inhibition promotes synaptogenesis in Drosophila and mammalian neurons." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118475, 2015 (Sample species: Human). Further details: Western Blotting

Ansell, Tang, Kurtin, Koenig, Inwards, Shah, Ziesmer, Feldman, Rao, Gupta, Erlichman, Witzig: "Temsirolimus and rituximab in patients with relapsed or refractory mantle cell lymphoma: a phase 2 study." in: The Lancet. Oncology, Vol. 12, Issue 4, pp. 361-8, 2011

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN153477) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN153477) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN153477) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 2)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN153477) Immunoblotting. Rabbit anti-AKT pS473 was used at a 1:200 dilution to detect phosphor...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN153477) Western Blot: Akt1 [p Ser473] Antibody [ABIN153477] - Lane 1: nuclear extract from ce...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN153477) Immunohistochemistry: Akt1 [p Ser473] Antibody [ABIN153477] - Tissue: human breast ca...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN153477) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody [ABIN153477] - Rabbit used at...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN153477) Western Blot: Akt1 [p Ser473] Antibody [ABIN153477] - Lane 1: AKT1 Recombinant Protei...
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN153477) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody [ABIN153477] - Tissu...