anti-Rat (Rattus) AKT antibody for Simple Western

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
pSer473
229
119
113
58
58
51
36
33
33
25
23
22
21
19
18
18
14
14
13
12
11
11
11
11
10
10
10
9
9
8
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1328
752
591
64
57
56
54
37
31
28
7
6
6
5
4
3
3
3
1
1
1
1
1
Host
Rabbit
1024
389
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
56
43
43
40
33
26
15
15
10
10
8
6
6
6
6
6
6
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
1215
617
594
267
202
202
138
90
64
38
32
26
12
11
10
4
3
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN4900619
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
31.67895 ABIN4899444 CyTOF FACS ICC SimWes WB Mouse IgG2b AA 2-480, pan Log in to see 281046 4

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473
(229), (119), (113), (58), (58), (51), (36), (33), (33), (25), (23), (22), (21), (19), (18), (18), (14), (14), (13), (12), (11), (11), (11), (11), (10), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1328), (752), (591), (64), (57), (56), (54), (37), (31), (28), (7), (6), (6), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Host Rabbit
(1024), (389), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(56), (43), (43), (40), (33), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
(1215), (617), (594), (267), (202), (202), (138), (90), (64), (38), (32), (26), (12), (11), (10), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 16 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN4900619) Images
Specificity Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
Purification Antigen and protein A Affinity-purified
Sterility 0.2 μm filtered
Immunogen Phosphopeptide containing human, mouse, and rat Akt1 S473 site
Isotype IgG
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN4900619) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Gene ID 207
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN4900619) Images back to top
Application Notes Western Blot 0.5 μg/mL
Simple Western 5 μg/mL
Immunohistochemistry 5-15 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN4900619) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-AKT antibody (ABIN4900619)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Werling, Reglodi, Banks, Salameh, Kovacs, Kvarik, Vaczy, Kovacs, Mayer, Danyadi, Lokos, Tamas, Toth, Biro, Atlasz: "Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents." in: Investigative ophthalmology & visual science, Vol. 57, Issue 15, pp. 6683-6691, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Budai, Tőzsér, Benkő: "Different dynamics of NLRP3 inflammasome-mediated IL‑1β production in GM-CSF- and M-CSF-differentiated human macrophages." in: Journal of leukocyte biology, Vol. 101, Issue 6, pp. 1335-1347, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Divakar, Ramana Reddy, Cosenza, Baker, Perumal, Antonelli, Brody, Akula, Parekh, Reddy: "Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas." in: Leukemia, Vol. 30, Issue 1, pp. 86-93, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Dicay, Hirota, Ronaghan, Peplowski, Zaheer, Carati, MacNaughton: "Interferon-γ suppresses intestinal epithelial aquaporin-1 expression via Janus kinase and STAT3 activation." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118713, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Marti, Koschwanez, Pesch, Blenn, Borel: "Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection." in: PLoS ONE, Vol. 9, Issue 7, pp. e102239, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Human).

von Roemeling, Radisky, Marlow, Cooper, Grebe, Anastasiadis, Tun, Copland: "Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4." in: Cancer research, Vol. 74, Issue 17, pp. 4796-810, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Mao, Xu, Li, Zhang, Yin, Liu: "The role of nuclear β-catenin accumulation in the Twist2-induced ovarian cancer EMT." in: PLoS ONE, Vol. 8, Issue 11, pp. e78200, 2013 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

He, Kang, Wu, Zhang, Wang, Gao, Zhang, Huang, Liu, Kou, Zhang, Gao: "An elaborate regulation of Mammalian target of rapamycin activity is required for somatic cell reprogramming induced by defined transcription factors." in: Stem cells and development, Vol. 21, Issue 14, pp. 2630-41, 2012 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Bifulco, Longanesi-Cattani, Franco, Pavone, Mugione, Di Carluccio, Masucci, Arra, Pirozzi, Stoppelli, Carriero: "Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion." in: PLoS ONE, Vol. 7, Issue 9, pp. e44806, 2012 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Maddigan, Truitt, Arsenault, Freywald, Allonby, Dean, Narendran, Xiang, Weng, Napper, Freywald: "EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 11, pp. 5983-94, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Deng, Han, Yan, Tian, Tao, Kang, Li: "Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 4, pp. 463-73, 2010 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).

Han, Wu, Deng, Tao, Guo, Tian, Kang, Zhang, Yan: "Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1225-35, 2010 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Wang, Haile, Comuzzi, Tien, Wang, Yong, Jelescu-Bodos, Blaszczyk, Vessella, Masri, Sadar: "Osteoblast-derived factors induce an expression signature that identifies prostate cancer metastasis and hormonal progression." in: Cancer research, Vol. 69, Issue 8, pp. 3433-42, 2009 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Han, Cui, Tao, Guo, Guo, Sun, Kang, Zhang, Yan, Li: "CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis." in: Experimental cell research, Vol. 315, Issue 19, pp. 3301-11, 2009 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Wang, Yue, Kim, Fu, Khuri, Sun: "Enhancing mammalian target of rapamycin (mTOR)-targeted cancer therapy by preventing mTOR/raptor inhibition-initiated, mTOR/rictor-independent Akt activation." in: Cancer research, Vol. 68, Issue 18, pp. 7409-18, 2008 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Nevo, Sagi-Assif, Meshel, Ben-Baruch, Jöhrer, Greil, Trejo, Kharenko, Feinmesser, Yron, Witz: "The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells." in: Cancer letters, Vol. 273, Issue 1, pp. 127-39, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN4900619) back to top
Supplier Images
Simple Western (SimWes) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 2)
Simple Western (SimWes) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 3)
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 4)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 5)