anti-Rat (Rattus) AKT antibody for Western Blotting

Recommended AKT Antibody

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
AA 445-480
184
94
88
74
52
50
45
30
30
26
21
18
18
18
17
17
15
14
13
13
12
12
12
12
12
11
10
9
8
7
7
7
6
6
6
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1337
751
627
57
57
54
35
33
31
29
6
6
6
5
4
3
3
3
2
1
1
1
Host
Rabbit
1118
257
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
64
38
33
32
29
26
19
19
19
19
18
18
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1121
608
606
230
169
143
136
64
55
38
32
18
12
11
8
5
3
3
2
1
1
1
1
1
Options
Successfully validated

Available images

Catalog No. ABIN725195
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
33.3578 ABIN6255359 ELISA ICC IF IHC WB Rabbit IgG pSer473 Polyclonal 19
32.450115 ABIN964561 IP IHC ELISA WB Mouse IgG1 kappa pSer473 17F6-B11 0
32.450115 ABIN5542263 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 1F7E10 0
32.450115 ABIN5542264 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 1F7G2 0
30.950115 ABIN94783 FM IHC (fp) IP ELISA WB Rabbit C-Term Polyclonal 4
30.950115 ABIN349616 IP IHC ELISA WB Mouse IgG1 kappa C-Term 18F10-E5 13
30.357798 ABIN6259867 ELISA ICC IF IHC IP WB Rabbit IgG Polyclonal 19
29.450115 ABIN3183239 ELISA IHC WB Rabbit IgG N-Term Polyclonal 0
28.857798 ABIN4899444 CyTOF FACS ICC SimWes WB Mouse IgG2b AA 2-480, pan 281046 7
27.950115 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region 17F6-B11 4
27.950115 ABIN1573993 ELISA WB Rabbit IgG pSer473 Polyclonal 1
27.357798 ABIN4900619 CyTOF FACS IHC SimWes WB Rabbit IgG pSer473 Polyclonal 21
26.450115 ABIN5596665 ELISA FACS FM IHC WB FITC Mouse IgG2a Internal Region 14E5-A2-B2-H9 0
26.450115 ABIN5596666 ELISA FACS FM IHC WB FITC Mouse IgG2a Internal Region 5E5-F5-D7 0
26.450115 ABIN5596664 ELISA FACS FM IHC WB Biotin Mouse IgG2a Internal Region 5E5-F5-D7 0
26.450115 ABIN5596663 ELISA FACS FM IHC WB Biotin Mouse IgG2a Internal Region 14E5-A2-B2-H9 0
26.450115 ABIN3181909 ELISA IHC WB Rabbit IgG pThr450 Polyclonal 0
26.450115 ABIN3183235 ELISA IHC WB Rabbit IgG Internal Region Polyclonal 0
24.950115 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region 17F6-B11 4
24.950115 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region 18F3-H11 4

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 445-480
(184), (94), (88), (74), (52), (50), (45), (30), (30), (26), (21), (18), (18), (18), (17), (17), (15), (14), (13), (13), (12), (12), (12), (12), (12), (11), (10), (9), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1337), (751), (627), (57), (57), (54), (35), (33), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(1118), (257), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(64), (38), (33), (32), (29), (26), (19), (19), (19), (19), (18), (18), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1121), (608), (606), (230), (169), (143), (136), (64), (55), (38), (32), (18), (12), (11), (8), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier

Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN725195) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human AKT1
Isotype IgG
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN725195) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Subcellular location: Cytoplasm, Nucleus

Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG

Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN725195) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN725195) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-AKT antibody (ABIN725195)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: testicular tissue

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Pig (Porcine)).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014 Method employed by authors: Western Blotting (WB)

Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, 2013 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: glioma tissue

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN725195) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 fo...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Lane 1: Mouse Placenta lysates;Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclon...
'Independent Validation' Badge
Lot Number 131113
Method validated Flow Cytometry
Positive Control MDA-MB-468 cells - lower expression
Notes A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.
Primary Antibody
  • Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
  • Catalog number: ABIN725195
  • Lot number: 131113
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Isotype Control Antibody
  • Antibody: Rabbit IgG
  • Lot number: 16
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Secondary Antibody
  • Antibody: Goat anti-rabbit IgG-FITC
  • Lot number: 115629
  • Dilution: 1:200 in 1X PBS containing 0.5% BSA
Controls
  • Positive control: MCF-7 cells
  • Low positive control: MDA-MB-468 cells
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-FITC to confirm no background signal produced from secondary antibody alone
Protocol
  • Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
  • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
  • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
  • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).
Experimental Notes Nothing to note.
Validation Images
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 1: Positive control (high expression) MCF7 cells. The red histogram is unstain...
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 2: Positive control (lower expression) MDA-MB-468 cells. The red histogram is ...