anti-Human AKT2 antibody for Fluorescence Microscopy

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • AKT serine/threonine kinase 2
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • v-akt murine thymoma viral oncogene homolog 2 L homeolog
  • AKT2
  • akt2
  • Akt2
  • akt2.L
Epitope
Internal Region
58
57
25
24
22
19
12
12
11
9
8
8
7
6
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
484
193
160
55
52
46
45
44
41
40
40
40
23
18
4
3
2
2
1
1
Host
Rat
348
105
18
14
3
2
Clonality
Monoclonal
Conjugate
This AKT2 antibody is conjugated to Biotin
24
21
19
19
18
16
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
422
268
199
94
63
59
36
36
31
19
13
11
7
4
4
3
3
2
1
1
Options
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Catalog No. ABIN5596672
$ 389.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5596673 ELISA FACS FM IHC WB Biotin Rat IgG2a Internal Region Log in to see 11F6-B2 0
1 ABIN5596674 ELISA FACS FM IHC WB FITC Rat IgG2a Internal Region Log in to see 16G11-E8 0
1 ABIN5596675 ELISA FACS FM IHC WB FITC Rat IgG2a Internal Region Log in to see 11F6-B2 0
1 ABIN5596669 ELISA FACS FM IHC WB APC Rat IgG2a Internal Region Log in to see 16G11-E8 0
1 ABIN5596670 ELISA FACS FM IHC WB APC Rat IgG2a Internal Region Log in to see 11F6-B2 0
1 ABIN5596676 ELISA FACS FM IHC WB PE Rat IgG2a Internal Region Log in to see 16G11-E8 0
1 ABIN5596677 ELISA FACS FM IHC WB PE Rat IgG2a Internal Region Log in to see 11F6-B2 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope Internal Region
(58), (57), (25), (24), (22), (19), (12), (12), (11), (9), (8), (8), (7), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(484), (193), (160), (55), (52), (46), (45), (44), (41), (40), (40), (40), (23), (18), (4), (3), (2), (2), (1), (1)
Host Rat
(348), (105), (18), (14), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT2 antibody is conjugated to Biotin
(24), (21), (19), (19), (18), (16), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
(422), (268), (199), (94), (63), (59), (36), (36), (31), (19), (13), (11), (7), (4), (4), (3), (3), (2), (1), (1)
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling Images
Cross-Reactivity (Details) Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
Purification Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein G chromatography.
Immunogen

Immunogen: Anti-AKT2 Antibody was produced in rat by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

Immunogen Type: Peptide

Clone 16G11-E8
Isotype IgG2a
Labeling Ratio 1-2
Plasmids, Primers & others

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT2 (AKT2 Antibody Abstract)
Background

Synonyms: AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT2 (RAT) PE conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Gene Name: AKT2

Gene ID 208
UniProt P31751
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Application Notes

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User optimized

Application Note: Anti-AKT2 BIOTIN Antibody is suitable for Flow Cytometry, ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

IF Microscopy Dilution: User optimized

Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL to 20 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Expiry Date 12 months

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Internal Region) antibody (Biotin) (ABIN5596672) Western Blot of Rat Anti-AKT2 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. ...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Internal Region) antibody (Biotin) (ABIN5596672) Western Blot of Rat anti-AKT2 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: ...
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Internal Region) antibody (Biotin) (ABIN5596672) Flow Cytometry of Rat anti-AKT2 antibody. Cells: Jurkat Cells. Stimulation: none. Pri...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Internal Region) antibody (Biotin) (ABIN5596672) Immunohistochemistry of Rat monoclonal anti AKT2 unconjugated Antibody in human breas...