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|Antigen||BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies|
|Conjugate||This BIM antibody is un-conjugated Alternatives|
ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Product Details anti-BIM AntibodyTarget Details BIM Application Details Handling Images
|Cross-Reactivity||Human, Mouse (Murine)|
|Cross-Reactivity (Details)||Cross reactivity with Bim [1C2C8] from other sources has not been determined.|
|Purification||Anti-Bim Monoclonal Antibody was Protein A purified from monospecific antiserum by immunoaffinity purification.|
Immunogen: Anti-Mouse monoclonal Bim antibody produced by repeated immunizations in mice with a recombinant protein corresponding to amino acids 2 to 198 of human Bim.
Immunogen Type: Peptide
|Plasmids, Primers & others|
Target Details BIMProduct Details anti-BIM Antibody Application Details Handling Images back to top
|Alternative Name||Bim (BCL2L11 Antibody Abstract)|
Synonyms: Bim Antibody [1C2C8] , BAM, BIM, BOD
Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
Gene Name: BCL2L11
|Pathways||PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-BIM Antibody Target Details BIM Handling Images back to top
Application Note: Anti-Bim Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 22 kDa in Western Blots of specific cell lysates and tissues.
ELISA Dilution: 1:10,000
Western Blot Dilution: 1 μg/mL
IF Microscopy Dilution: 20 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-BIM Antibody Target Details BIM Application Details Images back to top
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||RT,4 °C,-20 °C|
|Storage Comment||Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
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