anti-Human BIM antibody for Fluorescence Microscopy

Recommended BIM Antibody

Antigen
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
  • bim
  • BAM
  • BIM
  • BOD
  • 1500006F24Rik
  • Bim
  • Bod
  • BimL
  • BIMEL
  • BCL2-like 11
  • BCL2 like 11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl2l11
  • BCL2L11
  • Bcl2l11
Reactivity
Human
443
317
297
11
6
6
4
3
3
1
1
Host
Mouse
404
18
16
9
6
Clonality
Monoclonal
Conjugate
This BIM antibody is un-conjugated
25
15
12
12
11
11
10
10
10
10
10
10
6
6
6
6
6
6
6
6
6
6
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)
330
183
113
63
61
38
36
28
25
17
7
5
3
2
2
2
2
2
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Available images

Catalog No. ABIN6655897
$ 394.90
Plus shipping costs $45.00 and $22.60 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
10 ABIN6656866 ELISA FM WB Mouse IgG1 1C2H4 0
4 ABIN6656865 ELISA FM WB Rabbit IgG Internal Region Polyclonal 0

General

Antigen BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
Reactivity Human
(443), (317), (297), (11), (6), (6), (4), (3), (3), (1), (1)
Host Mouse
(404), (18), (16), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BIM antibody is un-conjugated
(25), (15), (12), (12), (11), (11), (10), (10), (10), (10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)
(330), (183), (113), (63), (61), (38), (36), (28), (25), (17), (7), (5), (3), (2), (2), (2), (2), (2)
Supplier

Product Details anti-BIM Antibody

Target Details BIM Application Details Handling Images
Cross-Reactivity Human, Mouse (Murine)
Cross-Reactivity (Details) Cross reactivity with Bim [1C2C8] from other sources has not been determined.
Purification Anti-Bim Monoclonal Antibody was Protein A purified from monospecific antiserum by immunoaffinity purification.
Immunogen

Immunogen: Anti-Mouse monoclonal Bim antibody produced by repeated immunizations in mice with a recombinant protein corresponding to amino acids 2 to 198 of human Bim.

Immunogen Type: Peptide

Clone 1C2C8
Isotype IgG1
Plasmids, Primers & others

Target Details BIM

Product Details anti-BIM Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bim (BCL2L11 Antibody Abstract)
Background

Synonyms: Bim Antibody [1C2C8] , BAM, BIM, BOD

Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Gene Name: BCL2L11

Gene ID 10018
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BIM Antibody Target Details BIM Handling Images back to top
Application Notes

Application Note: Anti-Bim Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 22 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions For Research Use only

Handling

Product Details anti-BIM Antibody Target Details BIM Application Details Images back to top
Format Liquid
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Images

Product Details anti-BIM Antibody Target Details BIM Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (ABIN6655897) Immunofluorescence of Bim Antibody [1C2C8] Immunofluorescence Microscopy of Mouse ant...
Western Blotting (WB) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (ABIN6655897) Western Blot of Bim Antibody Western Blot of Mouse anti-Bim antibody. Lane A: 5 ng of...
Immunocytochemistry (ICC) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (ABIN6655897) Immunocytochemistry of Bim Antibody Immunocytochemistry of Mouse anti-Bim antibody. T...