anti-Mouse (Murine) BIM antibody for Immunofluorescence

Recommended BIM Antibody

Antigen
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
  • bim
  • BAM
  • BIM
  • BOD
  • 1500006F24Rik
  • Bim
  • Bod
  • BimL
  • BIMEL
  • BCL2-like 11
  • BCL2 like 11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl2l11
  • BCL2L11
  • Bcl2l11
Reactivity
Human, Mouse (Murine), Rat (Rattus)
442
316
296
11
6
6
4
3
3
1
1
Host
Rabbit
402
18
17
9
6
Clonality
Polyclonal
Conjugate
This BIM antibody is un-conjugated
25
15
12
12
11
11
10
10
10
10
10
10
6
6
6
6
6
6
6
6
6
6
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
329
182
114
62
60
38
36
28
25
17
7
5
3
3
2
2
2
2
Options

Available images

Catalog No. ABIN361752
$ 453.70
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19.447392 ABIN5023241 ICC IF WB Atto 565 Rabbit Polyclonal 0
19.447392 ABIN5023244 ICC IF WB Atto 594 Rabbit Polyclonal 0
19.447392 ABIN5023262 ICC IF WB APC Rabbit Polyclonal 0
19.447392 ABIN5023253 ICC IF WB Atto 680 Rabbit Polyclonal 0
19.447392 ABIN5023250 ICC IF WB Atto 655 Rabbit Polyclonal 0
19.447392 ABIN5023268 ICC IF WB FITC Rabbit Polyclonal 0
19.447392 ABIN5023277 ICC IF WB PerCP Rabbit Polyclonal 0
19.447392 ABIN5023274 ICC IF WB PE-Atto 594 Rabbit Polyclonal 0
19.447392 ABIN5023238 ICC IF WB Atto 488 Rabbit Polyclonal 0
19.447392 ABIN5023235 ICC IF WB Atto 390 Rabbit Polyclonal 0
19.447392 ABIN5023256 ICC IF WB Atto 700 Rabbit Polyclonal 0
19.447392 ABIN5023247 ICC IF WB Atto 633 Rabbit Polyclonal 0
19.447392 ABIN5023271 ICC IF WB HRP Rabbit Polyclonal 0
19.447392 ABIN5023283 ICC IF WB Streptavidin Rabbit Polyclonal 0
19.447392 ABIN5023259 ICC IF WB Alkaline Phosphatase (AP) Rabbit Polyclonal 0
19.447392 ABIN5023265 ICC IF WB Biotin Rabbit Polyclonal 0
19.447392 ABIN5023280 ICC IF WB PE Rabbit Polyclonal 0
14.593272 ABIN2705596 IC IF IHC WB Rabbit pSer69 Polyclonal 0
13.447391 ABIN6929607 ICC IF WB Cy7,APC Rabbit Polyclonal 0
13.447391 ABIN6929608 ICC IF WB DyLight 350 Rabbit Polyclonal 0

General

Antigen BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(442), (316), (296), (11), (6), (6), (4), (3), (3), (1), (1)
Host Rabbit
(402), (18), (17), (9), (6)
Clonality
Polyclonal
Conjugate This BIM antibody is un-conjugated
(25), (15), (12), (12), (11), (11), (10), (10), (10), (10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(329), (182), (114), (62), (60), (38), (36), (28), (25), (17), (7), (5), (3), (3), (2), (2), (2), (2)
Pubmed 2 references available
Supplier

Product Details anti-BIM Antibody

Target Details BIM Application Details Handling References for anti-BIM antibody (ABIN361752) Images
Specificity Detects ~23 kDa.
Purification Protein A Purified
Immunogen Internal central amino acids of human Bim
Plasmids, Primers & others

Target Details BIM

Product Details anti-BIM Antibody Application Details Handling References for anti-BIM antibody (ABIN361752) Images back to top
Antigen
Alternative Name BIM/BOD (BCL2L11 Antibody Abstract)
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
Gene ID 10018
NCBI Accession NP_619527
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BIM Antibody Target Details BIM Handling References for anti-BIM antibody (ABIN361752) Images back to top
Application Notes
  • WB (1:400)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

2.5 μg/ml of SPC-164 was sufficient for detection of Bim in 20 μg of K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-BIM Antibody Target Details BIM Application Details References for anti-BIM antibody (ABIN361752) Images back to top
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C

References for anti-BIM antibody (ABIN361752)

Product Details anti-BIM Antibody Target Details BIM Application Details Handling Images back to top
Background publications

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998

OConnor, Strasser, OReilly, Hausmann, Adams, Cory, Huang: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998

Images

Product Details anti-BIM Antibody Target Details BIM Application Details Handling References for anti-BIM antibody (ABIN361752) back to top
Supplier Images
 image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (ABIN361752) Bim BOD (IN2), K562 Cells.
Western Blotting (WB) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (ABIN361752) Bim Bod A549 Cell lysate 10ug WB 1 in 1000.