anti-Rat (Rattus) BIM antibody for Enzyme Immunoassay

Recommended BIM Antibody

Antigen
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
  • bim
  • BAM
  • BIM
  • BOD
  • 1500006F24Rik
  • Bim
  • Bod
  • BimL
  • BIMEL
  • BCL2-like 11
  • BCL2 like 11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl2l11
  • BCL2L11
  • Bcl2l11
Reactivity
Human, Mouse (Murine), Rat (Rattus)
443
316
296
11
6
6
4
3
3
1
1
Host
Rabbit
403
18
17
9
6
Clonality
Polyclonal
Conjugate
This BIM antibody is un-conjugated
25
15
12
12
11
11
10
10
10
10
10
10
6
6
6
6
6
6
6
6
6
6
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
330
183
114
63
61
37
36
28
25
17
7
4
3
3
2
2
2
2
Options

Available images

Catalog No. ABIN265330
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN5554614 EIA IF WB Rabbit IgG Middle Region 2 Polyclonal 0
1 ABIN5551043 EIA IF WB Mouse IgG AA 2-198 1C2H4 0

General

Antigen BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(443), (316), (296), (11), (6), (6), (4), (3), (3), (1), (1)
Host Rabbit
(403), (18), (17), (9), (6)
Clonality
Polyclonal
Conjugate This BIM antibody is un-conjugated
(25), (15), (12), (12), (11), (11), (10), (10), (10), (10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(330), (183), (114), (63), (61), (37), (36), (28), (25), (17), (7), (4), (3), (3), (2), (2), (2), (2)
Supplier

Product Details anti-BIM Antibody

Target Details BIM Application Details Handling Images
Specificity This antibody detects endogenous levels of Bcl-2-like 11 / BIM-α/β protein (region surrounding Ala2).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE
Plasmids, Primers & others

Target Details BIM

Product Details anti-BIM Antibody Application Details Handling Images back to top
Antigen
Background Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS or BimL, implying a unique role for BimEL in cell survival signaling.Synonyms: BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS
Molecular Weight approx. 25 kDa
Gene ID 10018
NCBI Accession NP_001191035
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BIM Antibody Target Details BIM Handling Images back to top
Application Notes ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BIM Antibody Target Details BIM Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-BIM Antibody Target Details BIM Application Details Handling back to top
Supplier Images
 image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (ABIN265330) anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody
 image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (ABIN265330) anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (Image 2)