anti-Human CHUK antibody for ELISA

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase S homeolog
  • conserved helix-loop-helix ubiquitous kinase
  • chuk.S
  • CHUK
  • Chuk
  • chuk
Epitope
AA 646-745
47
34
16
15
15
15
11
11
10
10
9
8
8
8
7
7
7
6
6
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
352
163
113
20
12
11
10
7
6
5
5
4
2
1
Host
Mouse
278
75
14
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
9
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
325
160
119
75
60
46
39
29
26
19
9
5
3
3
2
2
1
1
1
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Catalog No. ABIN393762
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.028485 ABIN560377 IF PLA ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 1F8 0
12.028485 ABIN560378 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 4B8 0
12.028485 ABIN560376 IF ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 4D11 0
1 ABIN1531285 IHC ELISA WB Rabbit IgG AA 151-200, pSer176 Log in to see Polyclonal 3
1 ABIN4324237 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 2
1 ABIN4324257 ELISA WB Goat C-Term Log in to see Polyclonal 1
1 ABIN1531873 IHC ELISA WB Rabbit IgG AA 15-64, pThr23 Log in to see Polyclonal 0
1 ABIN1532904 IHC ELISA WB Rabbit IgG AA 15-64 Log in to see Polyclonal 0
1 ABIN2869330 ELISA WB Mouse IgG1 AA 500-590 Log in to see 7C10B6-1A10A10 0
1 ABIN3185147 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal 0
1 ABIN3182039 ELISA IHC WB Rabbit IgG pThr23 Log in to see Polyclonal 0
1 ABIN4880258 ELISA WB Mouse IgG1 Log in to see 3G12 0
1 ABIN4880259 ELISA FACS ICC WB Mouse IgG1 Log in to see 3G12H9 0
1 ABIN466713 ELISA WB Mouse IgG1 AA 500-590 Log in to see 7C10B6 0
1 ABIN364294 ELISA IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1846074 ELISA FACS IF/ICC WB Mouse IgG1 Log in to see 0
1 ABIN4324236 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN598611 IF ELISA WB Mouse IgG1, kappa AA 646-745 Log in to see 4B8 0
1 ABIN598612 IF ELISA WB Mouse IgG1, kappa AA 646-745 Log in to see 2G4 0
1 ABIN1497518 ELISA WB Mouse IgG1 Log in to see 3G12 0

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope AA 646-745
(47), (34), (16), (15), (15), (15), (11), (11), (10), (10), (9), (8), (8), (8), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(352), (163), (113), (20), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Mouse
(278), (75), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (9), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(325), (160), (119), (75), (60), (46), (39), (29), (26), (19), (9), (5), (3), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN393762) Images
Specificity CHUK (NP_001269, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: CHUK (NP_001269, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 4B8
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK antibody (ABIN393762) Images back to top
Antigen
Alternative Name CHUK (CHUK Antibody Abstract)
Background

Other Names: Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16, CHUK, IKKA, TCF16

Gene Description: conserved helix-loop-helix ubiquitous kinase

Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq

Molecular Weight 84654 DA
Gene ID 1147
NCBI Accession NP_001269
UniProt O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK antibody (ABIN393762) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK antibody (ABIN393762) Images back to top
Concentration 0.37 mg/mL
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-CHUK antibody (ABIN393762)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Background publications

Li, Morgan, Choksi, Zhang, Kim, Liu: "MicroRNAs modulate the noncanonical transcription factor NF-kappaB pathway by regulating expression of the kinase IKKalpha during macrophage differentiation." in: Nature immunology, Vol. 11, Issue 9, pp. 799-805, 2010

Rotman, Koh, Zmuda, Kleiner, Liang: "The association of genetic variability in patatin-like phospholipase domain-containing protein 3 (PNPLA3) with histological severity of nonalcoholic fatty liver disease." in: Hepatology (Baltimore, Md.), Vol. 52, Issue 3, pp. 894-903, 2010

Jugessur, Shi, Gjessing, Lie, Wilcox, Weinberg, Christensen, Boyles, Daack-Hirsch, Nguyen, Christiansen, Lidral, Murray: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Olson, Wang, Pankratz, Fredericksen, Vachon, Vierkant, Cerhan, Couch: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN393762) back to top
Supplier Images
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (ABIN393762) Immunofluorescence of monoclonal antibody to CHUK on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (ABIN393762) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (Image 2)
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (ABIN393762) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (ABIN393762) CHUK monoclonal antibody (M03), clone 4B8 Western Blot analysis of CHUK expression in...
Immunohistochemistry (IHC) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (ABIN393762) Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded h...
Immunofluorescence (IF) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (ABIN393762) Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentrati...
ELISA image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (ABIN393762) Detection limit for recombinant GST tagged CHUK is 0.03 ng/ml as a capture antibody.