anti-Human CHUK antibody for Western Blotting

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase S homeolog
  • conserved helix-loop-helix ubiquitous kinase
  • chuk.S
  • CHUK
  • Chuk
  • chuk
Reactivity
Human
354
165
114
20
12
11
10
7
6
5
5
4
2
1
Host
Rabbit
281
75
13
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
9
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
327
157
119
71
61
43
43
29
27
17
9
5
3
3
2
2
2
1
1
Options
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Available images

Catalog No. ABIN3022825
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
32.699123 ABIN252647 ChIP FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231 74
26.68801 ABIN969056 ICC FACS ELISA WB Mouse IgG1 Log in to see 3G12H9 1
23.68801 ABIN3019713 IHC WB Rabbit pThr23 Log in to see Polyclonal 1
22.18801 ABIN931520 IF ELISA WB Mouse IgG1 Log in to see 3G12H9 0
20.699125 ABIN4324239 CyTOF FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231 43
20.68801 ABIN969054 ELISA WB Mouse IgG1 AA 500-590 Log in to see 7C10B6 2
20.68801 ABIN969055 ELISA WB Mouse IgG1 Log in to see 3G12 1
19.730314 ABIN393762 ELISA IF IHC WB Mouse IgG1 kappa AA 646-745 Log in to see 4B8 5
19.730314 ABIN6256173 ELISA ICC IF IHC WB Rabbit IgG pSer176, pSer177 Log in to see Polyclonal 0
19.730314 ABIN393623 IF IHC WB Mouse IgG1 kappa AA 646-745 Log in to see 2G4 0
19.730314 ABIN560379 IF IHC (p) PLA ELISA WB Mouse IgG1 kappa AA 646-745 Log in to see 2G4 0
19.18801 ABIN3182039 ELISA IHC WB Rabbit IgG pThr23 Log in to see Polyclonal 0
19.18801 ABIN3185147 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal 0
18.457695 ABIN100660 WB Rabbit C-Term Log in to see Polyclonal 0
18.230314 ABIN6673330 IHC WB Rabbit IgG Log in to see Polyclonal 3
16.957695 ABIN965896 ELISA WB Mouse IgG1 AA 500-590 Log in to see 7C10B6-1A10A10 3
16.730314 ABIN1871859 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
16.730314 ABIN560378 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 646-745 Log in to see 4B8 0
16.18801 ABIN931383 ELISA WB Mouse IgG1 AA 500-590 Log in to see 7C10B6-1A10A10 0
16.18801 ABIN931518 IF ELISA WB Mouse IgG1 Log in to see 3G12 0

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human
(354), (165), (114), (20), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Rabbit
(281), (75), (13), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (9), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(327), (157), (119), (71), (61), (43), (43), (29), (27), (17), (9), (5), (3), (3), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN3022825) Images
Purification Affinity purification
Immunogen A synthetic peptide of human CHUK
Isotype IgG
Plasmids, Primers & others

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK antibody (ABIN3022825) Images back to top
Antigen
Alternative Name CHUK (CHUK Antibody Abstract)
Background The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins. Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkappaB. The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunits of the kinase and IKKgamma serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKbeta (Ser176 and Ser180 in IKKalpha), which causes conformational changes, resulting in kinase activation.
Synonyms: CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Gene ID 1147
UniProt O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK antibody (ABIN3022825) Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK antibody (ABIN3022825) Images back to top
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

References for anti-CHUK antibody (ABIN3022825)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Product cited in:

Wang, Wang, Liu, Wang, Xu, Liu, Zhu, Chen, Situ, Lin: "Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages." in: International journal of molecular medicine, Vol. 41, Issue 1, pp. 340-352, 2018 Method employed by authors: Western Blotting (WB)

Gao, Yang, Yuan, Huang, Xu, Mao, Yuan, Bi: "TNFα-YAP/p65-HK2 axis mediates breast cancer cell migration." in: Oncogenesis, Vol. 6, Issue 9, pp. e383, 2017 Method employed by authors: Western Blotting (WB)

Huang, Dong, Sui, Hu, Xiong, Liao, Zhang: "MiR-223 suppresses endometrial carcinoma cells proliferation by targeting IGF-1R." in: American journal of translational research, Vol. 6, Issue 6, pp. 841-9, 2015 Method employed by authors: Western Blotting (WB)

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN3022825) back to top
Supplier Images
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN3022825) Western blot analysis of extracts of various cell lines, using CHUK antibody.
Immunofluorescence (IF) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN3022825) Immunofluorescence analysis of A549 cell using CHUK antibody. Blue: DAPI for nuclear ...
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN3022825) Western blot analysis of extracts of various cell lines, using CHUK antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN3022825) Immunohistochemistry of paraffin-embedded rat brain using CHUK antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN3022825) Immunohistochemistry of paraffin-embedded mouse kidney using CHUK antibody.
Immunofluorescence (IF) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN3022825) Immunofluorescence analysis of A549 cells using CHUK antibody.