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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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13.98401 | ABIN4324239 | CyTOF FACS ICC IF IHC (fro) IHC (p) IP SimWes WB | Mouse | IgG1 kappa | Log in to see | 14A231 | 43 | |||
1 | ABIN959200 | FACS IHC (p) IP WB | Mouse | IgG1, kappa | Log in to see | 14A231 | 0 | |||
1 | ABIN4324231 | ChIP FACS ICC IF IHC (fro) IHC (p) IP SimWes WB | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | |||
1 | ABIN4324240 | FACS | APC | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324242 | FACS ICC IF IHC (fro) IHC (p) WB | DyLight 650 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324246 | FACS ICC IF IHC (fro) IHC (p) WB | DyLight 755 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324249 | FACS ICC IF IHC (fro) IHC (p) WB | DyLight 550 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324254 | FACS ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 647 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324243 | FACS ICC IF IHC (fro) IHC (p) WB | DyLight 680 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324247 | FACS IHC (p) IP WB | PerCP | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324253 | FACS ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 405 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324251 | FACS ICC IF IHC (fro) IHC (p) WB | DyLight 405 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324255 | FACS ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 700 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324256 | FACS ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 488 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324248 | FACS | PE | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324244 | FACS ICC IF IHC (fro) IHC (p) WB | DyLight 488 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4324250 | FACS ICC IF IHC (fro) IHC (p) WB | DyLight 350 | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 | ||
1 | ABIN4995164 | FACS ICC IF IHC (fro) IHC (p) | FITC | Mouse | IgG1 kappa | Log in to see | 14A231 | 0 |
General |
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Antigen | conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies |
Reactivity | Human, Mouse (Murine), Primate, Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This CHUK antibody is un-conjugated Alternatives |
Application |
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
Alternatives
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73 references available |
Supplier | Log in to see |
Product Details anti-CHUK AntibodyTarget Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN252647) Images |
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Specificity | An 85 kDa band should be observed. |
Purification | Protein G purified |
Immunogen | This antibody was raised against a His-tagged full-length human IKK alpha protein. |
Clone | 14A231 |
Isotype | IgG1 kappa |
Target Details CHUKProduct Details anti-CHUK Antibody Application Details Handling References for anti-CHUK antibody (ABIN252647) Images back to top |
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Antigen | |
Alternative Name | IKK alpha (CHUK Antibody Abstract) |
Background | Gene Symbol: CHUK |
Gene ID | 1147 |
UniProt | O15111 |
Pathways | PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades |
Application DetailsProduct Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK antibody (ABIN252647) Images back to top |
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Application Notes | Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry (Intracellular): 0.25-0.5 μg/10^6 cells, Immunocytochemistry/Immunofluorescence 1:10, Immunoprecipitation 1-2 μg/mL, Immunohistochemistry-Paraffin 5 μg/mL, Immunohistochemistry-Frozen, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP)Use in Flow Intracellular reported in scientific literature (PMID 24804954) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25133425). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in chromatin immunoprecipitation reported in scientific literature (PMID 27705798). |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK antibody (ABIN252647) Images back to top |
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Format | Liquid |
Concentration | 1 mg/mL |
Buffer |
PBS - BSA free Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | 4 °C,-20 °C |
Storage Comment | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
References for anti-CHUK antibody (ABIN252647)Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top |
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Product cited in: |
Diamanti, Gupta, Bennecke, De Oliveira, Ramakrishnan, Braczynski, Richter, Beli, Hu, Saleh, Mittelbronn, Dikic, Greten: "IKKα controls ATG16L1 degradation to prevent ER stress during inflammation." in: The Journal of experimental medicine, Vol. 214, Issue 2, pp. 423-437, 2017 Method employed by authors: Western Blotting (WB) Zhu, Willette-Brown, Song, Lomada, Song, Xue, Gray, Zhao, Davis, Sun, Zhang, Wu, Zhan, Richie, Hu: "Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis." in: Cell host & microbe, Vol. 21, Issue 4, pp. 478-493.e7, 2017 Todoric, Antonucci, Di Caro, Li, Wu, Lytle, Dhar, Banerjee, Fagman, Browne, Umemura, Valasek, Kessler, Tarin, Goggins, Reya, Diaz-Meco, Moscat, Karin: "Stress-Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas." in: Cancer cell, Vol. 32, Issue 6, pp. 824-839.e8, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human). Vincendeau, Hadian, Messias, Brenke, Halander, Griesbach, Greczmiel, Bertossi, Stehle, Nagel, Demski, Velvarska, Niessing, Geerlof, Sattler, Krappmann: "Inhibition of Canonical NF-κB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction." in: Scientific reports, Vol. 6, pp. 18934, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting Shintaku, Peterson, Talbert, Gu, Ladner, Williams, Mousavi, Wang, Sartorelli, Guttridge: "MyoD Regulates Skeletal Muscle Oxidative Metabolism Cooperatively with Alternative NF-κB." in: Cell reports, Vol. 17, Issue 2, pp. 514-526, 2016 Method employed by authors: Western Blotting (WB) Alameda, Gaspar, Ramírez, Navarro, Page, Suárez-Cabrera, Fernández, Mérida, Paramio, García-Fernández, Fernández-Aceñero, Casanova: "Deciphering the role of nuclear and cytoplasmic IKKα in skin cancer." in: Oncotarget, Vol. 7, Issue 20, pp. 29531-47, 2016 Method employed by authors: Immunocytochemistry (ICC), Immunofluorescence (IF) (Sample species: Human). Alameda, Navarro, Ramírez, Page, Suárez-Cabrera, Moreno-Maldonado, Paramio, del Carmen Fariña, Del Río, Fernández-Aceñero, Bravo, de Los Llanos Casanova: "IKKα regulates the stratification and differentiation of the epidermis: implications for skin cancer development." in: Oncotarget, Vol. 7, Issue 47, pp. 76779-76792, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB) (Sample species: Human). Fang, Jeong, Long, Park, Wang, Shuai, Wei, Li, Li, Zhang, Duran, Lo, Tsao, Glaser, Luo, Feng, Tian, Luo: "IKKα-mediated biogenesis of miR-196a through interaction with Drosha regulates the sensitivity of cancer cells to radiotherapy." in: Cell death and differentiation, Vol. 23, Issue 9, pp. 1471-82, 2016 Mihalas, Meffert: "IKK kinase assay for assessment of canonical NF-κB activation in neurons." in: Methods in molecular biology (Clifton, N.J.), Vol. 1280, pp. 61-74, 2015 Remouchamps, Dejardin: "Methods to assess the activation of the alternative (noncanonical) NF-κB pathway by non-death TNF receptors." in: Methods in molecular biology (Clifton, N.J.), Vol. 1280, pp. 103-19, 2015 Ren, Takahashi, Liu, Loughran, Sun, Wang, Cheng: "HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains." in: Oncogene, Vol. 34, Issue 3, pp. 334-45, 2015 (Sample species: Human). Further details: Western Blotting Gray, McCorkell, Chunduru, McKinlay, May: "Negative feedback regulation of NF-κB-inducing kinase is proteasome-dependent but does not require cellular inhibitors of apoptosis." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 341-6, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting Richardson, Hammond, Coulombe, Saloranta, Nousiainen, Salonen, Berry, Hanley, Headon, Karikoski, Dixon: "Periderm prevents pathological epithelial adhesions during embryogenesis." in: The Journal of clinical investigation, Vol. 124, Issue 9, pp. 3891-900, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections) Gray, Remouchamps, McCorkell, Solt, Dejardin, Orange, May: "Noncanonical NF-κB signaling is limited by classical NF-κB activity." in: Science signaling, Vol. 7, Issue 311, pp. ra13, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation Pradhan, Pratheek, Garai, Kumar, Meena, Ghosh, Singh, Kumari, Chandrashekar, Goswami, Chattopadhyay, Kar, Maiti: "Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells." in: Cell biology international, Vol. 38, Issue 10, pp. 1118-31, 2014 (Sample species: Human). Further details: Flow Cytometry,Immunochromatography Das, Manna, Sinha, Datta, Das, Chakraborty, Ghosh, Saha, Dey: "Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model." in: PLoS ONE, Vol. 9, Issue 5, pp. e97599, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting Buhrmann, Busch, Shayan, Shakibaei: "Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22048-62, 2014 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation Schwitalla, Fingerle, Cammareri, Nebelsiek, Göktuna, Ziegler, Canli, Heijmans, Huels, Moreaux, Rupec, Gerhard, Schmid, Barker, Clevers, Lang, Neumann, Kirchner, Taketo, van den Brink, Sansom, Arkan et al.: "Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties. ..." in: Cell, Vol. 152, Issue 1-2, pp. 25-38, 2013 Further details: Western Blotting Mihalas, Araki, Huganir, Meffert: "Opposing action of nuclear factor ?B and Polo-like kinases determines a homeostatic end point for excitatory synaptic adaptation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 42, pp. 16490-501, 2013 Further details: Immunoprecipitation Bimonte, Barbieri, Palma, Luciano, Rea, Arra: "Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer." in: BioMed research international, Vol. 2013, pp. 810423, 2013 (Sample species: Human). Further details: Western Blotting |
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