anti-Mouse (Murine) CHUK antibody for Western Blotting

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase S homeolog
  • conserved helix-loop-helix ubiquitous kinase
  • chuk.S
  • CHUK
  • Chuk
  • chuk
Epitope
pSer176, pSer177
43
34
16
15
15
15
11
10
10
10
10
9
9
8
8
7
7
6
6
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
348
163
112
20
12
11
10
7
6
5
5
4
2
1
Host
Rabbit
277
73
13
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
9
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
321
154
115
71
59
43
39
29
27
20
9
5
3
3
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN6256173
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.39645 ABIN3185147 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal 0
19.39645 ABIN3182039 ELISA IHC WB Rabbit IgG pThr23 Log in to see Polyclonal 0
13.820231 ABIN6255637 ELISA ICC IF IHC WB Rabbit IgG pThr23 Log in to see Polyclonal 0
13.820231 ABIN2973335 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
12.576219 ABIN1842387 IHC WB Rabbit IgG pThr23 Log in to see Polyclonal 0
12.576219 ABIN5623251 IP WB Mouse IgG1 kappa Log in to see 3A11 0
10.820231 ABIN3043812 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal 0
10.820231 ABIN6262511 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.820231 ABIN5967175 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.820231 ABIN3030568 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal 0
10.820231 ABIN2426593 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN364294 ELISA IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
8.5 ABIN681508 IF (p) IHC (p) WB Rabbit IgG AA 555-585 Log in to see Polyclonal 2
7.8202314 ABIN2707589 IHC WB Rabbit N-Term, pSer23 Log in to see Polyclonal 0
7.8202314 ABIN2707590 IHC WB Rabbit N-Term Log in to see Polyclonal 0
7.8202314 ABIN1531285 IHC ELISA WB Rabbit IgG AA 151-200, pSer176 Log in to see Polyclonal 3
7.8202314 ABIN1532904 IHC ELISA WB Rabbit IgG AA 15-64 Log in to see Polyclonal 0
7.8202314 ABIN1531873 IHC ELISA WB Rabbit IgG AA 15-64, pThr23 Log in to see Polyclonal 0
7.8202314 ABIN1870076 IHC WB Rabbit IgG pThr23 Log in to see Polyclonal 0
7.8202314 ABIN498244 IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope pSer176, pSer177
(43), (34), (16), (15), (15), (15), (11), (10), (10), (10), (10), (9), (9), (8), (8), (7), (7), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(348), (163), (112), (20), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Rabbit
(277), (73), (13), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (9), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(321), (154), (115), (71), (59), (43), (39), (29), (27), (20), (9), (5), (3), (3), (2), (2), (1), (1)
Supplier Log in to see

Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling Images
Specificity Phospho-IKK- alpha (Ser176) /IKK- beta (Ser177) Antibody detects endogenous levels of IKK- alpha /IKK- beta only when phosphorylated at Serine 177
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human IKK- alpha /IKK- beta around the phosphorylation site of Serine 177
Isotype IgG
Plasmids, Primers & others

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKK alpha (CHUK Antibody Abstract)
Background

Description: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor (PubMed:15084260).

Gene: CHUK

Molecular Weight 85kDa
Gene ID 1147
UniProt O15111, O14920
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibody (ABIN6256173) ABIN6267231 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilize...
Immunohistochemistry (IHC) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibody (ABIN6256173) ABIN6267231 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue...
Immunohistochemistry (IHC) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibody (ABIN6256173) ABIN6267231 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissu...
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibody (ABIN6256173) Western blot analysis of TIMP4 expression in NIH-3T3 whole cell lysates,The lane on t...
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibody (ABIN6256173) Western blot analysis of IKK alpha /IKK beta phosphorylation expression in TNF treate...
Immunohistochemistry (IHC) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibody (ABIN6256173) ABIN6267231 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue...