anti-Mouse (Murine) Cyclin D1 antibody for Immunoprecipitation

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ccnd1b
  • prad1
  • ccnd1
  • ccnd1a
  • bcl1
  • d11s287e
  • u21b31
  • CCND1
  • cyclin D1
  • cyclin D1 S homeolog
  • cyclin D1 L homeolog
  • CCND1
  • Ccnd1
  • ccnd1
  • ccnd1.S
  • ccnd1.L
Epitope
full length
75
40
24
18
14
12
11
10
9
8
8
6
6
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
469
239
227
44
21
20
19
17
14
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
325
203
19
4
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
26
23
15
10
8
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
461
208
126
126
109
108
102
80
51
12
8
8
7
6
5
3
1
1
1
1
Options
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Catalog No. ABIN967430
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967539 IHC (f) IHC (fro) ICS IP WB Mouse IgG2a full length Log in to see DCS-6 4
1 ABIN1106871 FACS IHC (p) IP WB Mouse IgG2a Log in to see 5D4 3
1 ABIN967431 FACS IP IHC WB Mouse IgG1 full length Log in to see G124-326 5
1 ABIN115708 IHC (p) IP WB Rabbit IgG C-Term Log in to see SP4
1 ABIN2736604 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN800381 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1077956 ICC IHC IP WB Rabbit IgG AA 31-295 Log in to see Polyclonal
1 ABIN1501962 ICC IHC (fro) IHC (p) IP WB Magnetic Particles Mouse IgG2a Log in to see CY-D1
1 ABIN1824080 FACS IP WB Mouse IgG2a Log in to see DCS-6
1 ABIN211137 Func ICC IP WB Rabbit IgG AA 285-295 Log in to see Polyclonal
1 ABIN1495157 IP WB Magnetic Particles Rabbit N-Term Log in to see Polyclonal
1 ABIN4301541 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
1 ABIN4301539 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
1 ABIN4301540 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
1 ABIN4301565 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see SPM587
1 ABIN4301577 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG2a kappa Log in to see SPM587
1 ABIN4301564 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see SPM587
1 ABIN4301568 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a kappa Log in to see SPM587
1 ABIN4301566 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see SPM587
1 ABIN4301570 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2a kappa Log in to see SPM587

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope full length
(75), (40), (24), (18), (14), (12), (11), (10), (9), (8), (8), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(469), (239), (227), (44), (21), (20), (19), (17), (14), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(325), (203), (19), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(26), (23), (15), (10), (8), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(461), (208), (126), (126), (109), (108), (102), (80), (51), (12), (8), (8), (7), (6), (5), (3), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN967430) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant full-length human cyclin D1
Clone G124-326
Isotype IgG1

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 antibody (ABIN967430) Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate the progression of the cell cycle. The synthesis and degradation of cyclins is tightly controlled in a cell cycle specific manner. Several classes of cyclins (A-E) have been described. Cyclins have been placed into functional groups as follows: Group 1 (cyclins A, B, D1, D2, D3, E and F) functions primarily in cell cycle reguation, Group 2 (cyclins C and H) also plays a role in transcriptional regulation, Group 3 (cyclins G1, G2 and I) may play a role distinct from other cyclins. Specific substrates for cyclin-cdk complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T Ag), tumor suppressor genes (Rb and p53), and others. The D-type cyclins are involved in regulating the passage of mammalian cells through G1. The reduced molecular weights of cyclin D1 is ~36 kD. G124-326 recognizes human cyclin D1 and cross-reacts with the mouse homolog (Cyl1) of human cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human cyclin D1 was used as immunogen. The antibody was originally evaluated by ELISA, western blot analysis and immunohistochemistry of frozen and paraffin-embedded tissue sections.
Molecular Weight 36 kDa
Research Area Cancer, Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 antibody (ABIN967430) Images back to top
Application Notes D-type cyclins are differentially expressed in distinct cell types, therefore cell types which have been documented to express high levels of a given D-type cyclin are suggested as positive controls. WI-38 human diploid fibroblasts (ATCC CCL 75), and U-118 human glioblastoma cells (ATCC HTB 15) are suggested for detecting cyclin D1. For flow cytometric analysis, T-47D (ATCC HTB 133) and MCF7 (ATCC HTB 22) human breast carcinoma cell lines are suggested as positive controls. G124-326 can also be used for immunohistochemistry of frozen and paraffin-embedded tissue sections (5-20 µg/ml). For immunohistochemistry, normal colon tissue and the HT-29 colon adenocarcinoma cells (ATCC HTB 38) are suggested as positive controls for detecting cyclin D1. Scattered cells are stained, and staining is nuclear and/or cytoplasmic. Shapiro et al (1995) found many positive cells in non-small cell lung cancer and small lung cancer specimens that overexpress cyclin D1. This suggests that they may also be useful as positive controls for immunohistochemical detection of cyclin D1.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 antibody (ABIN967430) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Cyclin D1 antibody (ABIN967430)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Product cited in:

Bates, Rowan, Vousden: "Characterisation of human cyclin G1 and G2: DNA damage inducible genes." in: Oncogene, Vol. 13, Issue 5, pp. 1103-9, 1996

Gong, Traganos, Darzynkiewicz: "Threshold expression of cyclin E but not D type cyclins characterizes normal and tumour cells entering S phase." in: Cell proliferation, Vol. 28, Issue 6, pp. 337-46, 1995

Shapiro, Edwards, Kobzik, Godleski, Richards, Sugarbaker, Rollins: "Reciprocal Rb inactivation and p16INK4 expression in primary lung cancers and cell lines." in: Cancer research, Vol. 55, Issue 3, pp. 505-9, 1995

Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN967430) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin D1 (CCND1) (full length) antibody (ABIN967430) Western blot analysis of Cyclin D1. Lane 1, WI-38 human diploid fibroblast cell lysat...
 image for anti-Cyclin D1 (CCND1) (full length) antibody (ABIN967430) anti-Cyclin D1 (CCND1) (full length) antibody (Image 2)