anti-Primate Cyclin D1 antibody for Immunocytochemistry

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ccnd1b
  • prad1
  • ccnd1
  • ccnd1a
  • bcl1
  • d11s287e
  • u21b31
  • CCND1
  • cyclin D1
  • cyclin D1 S homeolog
  • cyclin D1 L homeolog
  • cyclin D2
  • CCND1
  • Ccnd1
  • ccnd1
  • ccnd1.S
  • ccnd1.L
  • CCND2
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
460
226
214
43
19
19
18
17
14
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
317
200
19
5
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
25
23
15
10
8
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
461
213
131
120
102
101
97
79
51
12
8
7
6
5
4
3
1
1
1
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Catalog No. ABIN4301539
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4301540 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301541 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301564 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301568 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301569 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301573 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301576 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301566 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301571 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301577 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301578 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301574 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301563 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301565 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301570 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301555 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a kappa Log in to see DCS-6 0
1 ABIN4301534 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a kappa Log in to see DCS-6 0
1 ABIN4301572 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB HRP Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301562 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301561 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587 0

General

Antigen Cyclin D1 (CCND1) Antibodies
Reactivity Human, Mouse (Murine), Primate, Rat (Rattus)
(460), (226), (214), (43), (19), (19), (18), (17), (14), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(317), (200), (19), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(25), (23), (15), (10), (8), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(461), (213), (131), (120), (102), (101), (97), (79), (51), (12), (8), (7), (6), (5), (4), (3), (1), (1), (1)
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling Images
Specificity Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Purification Protein A purified
Immunogen Human recombinant full length cyclin D1 protein
Clone SPM587
Isotype IgG2a kappa

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Gene Symbol: CCND1
Molecular Weight Theoretical MW: 36 kDa
Gene ID 595
UniProt P24385, P25322, P39948
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
Application Notes Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1 - 2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mLHuman Chromosome Location: 11q13.2 ELISA and Biological Function Blockade (Order Ab without BSA & Azide) Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. ELISA (For coating, order NBP2-34816 (the BSA free version of NBP2-32840))
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.