anti-Rat (Rattus) Cyclin D1 antibody for Flow Cytometry

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ccnd1b
  • prad1
  • ccnd1
  • ccnd1a
  • bcl1
  • d11s287e
  • u21b31
  • CCND1
  • cyclin D1
  • cyclin D1 S homeolog
  • cyclin D1 L homeolog
  • cyclin D2
  • CCND1
  • Ccnd1
  • ccnd1
  • ccnd1.S
  • ccnd1.L
  • CCND2
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
470
236
225
44
20
20
19
17
14
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
323
205
19
5
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
26
23
15
10
8
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
472
218
130
124
110
104
103
81
51
12
8
8
7
6
5
3
1
1
1
1
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Catalog No. ABIN3026634
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.951413 ABIN3026632 FACS IF PE Mouse IgG2a, kappa Log in to see CCND1-809 0
13.951413 ABIN3024574 FACS IF IHC WB Mouse IgG2a, kappa Log in to see SPM587 0
1 ABIN574432 ICC IHC (fro) FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see CD1-1 0
1 ABIN2471067 ICC FACS IP IHC ELISA WB Mouse IgG1 Log in to see CD1-1 0
1 ABIN255827 FACS IHC (fro) IHC (p) IP IHC ELISA WB Mouse IgG1 Log in to see CD1-1 0
1 ABIN1723570 ICC IHC (fro) FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see CD1-1 0
1 ABIN4301567 FACS APC Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN199058 FACS IF IHC WB FITC Mouse IgG2a, kappa Log in to see 0
1 ABIN1824080 FACS IP WB Mouse IgG2a Log in to see DCS-6 0
1 ABIN2859383 FACS IF Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN200701 FACS IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 Log in to see 0
1 ABIN2618272 ELISA FACS IF IHC (fro) IHC (p) IP WB Mouse IgG2a, kappa Log in to see 0
1 ABIN4301539 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301540 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301541 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301564 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301568 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301569 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301573 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301576 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG2a kappa Log in to see SPM587 0

General

Antigen Cyclin D1 (CCND1) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(470), (236), (225), (44), (20), (20), (19), (17), (14), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(323), (205), (19), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(26), (23), (15), (10), (8), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(472), (218), (130), (124), (110), (104), (103), (81), (51), (12), (8), (8), (7), (6), (5), (3), (1), (1), (1), (1)
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling Images
Characteristics This antibody recognizes a protein of 36 kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Purification Protein G affinity chromatography
Immunogen Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.
Clone DCS-6
Isotype IgG2a, kappa

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background This antibody recognizes a protein of 36 kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Gene ID 595
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Cyclin D1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Cyclin D1 (CCND1) antibody (ABIN3026634) FACS testing of Jurkat cells: Black=cells alone; Green=isotype control; Red=Cyclin D1...
Flow Cytometry (FACS) image for anti-Cyclin D1 (CCND1) antibody (ABIN3026634) FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=Cyclin D1 ...
Immunohistochemistry (IHC) image for anti-Cyclin D1 (CCND1) antibody (ABIN3026634) IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear s...
Western Blotting (WB) image for anti-Cyclin D1 (CCND1) antibody (ABIN3026634) Western blot testing of human and mouse samples with DCS-6 mAb.