anti-Primate Huntingtin antibody for Western Blotting

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • HTT
  • hd
  • ZHD
  • CG9995
  • Dmel\\CG9995
  • HD
  • Hsap\\HD
  • Htt
  • dHtt
  • dhtt
  • SLC6A4
  • huntington
  • it15
  • htt
  • IT15
  • AI256365
  • C430023I11Rik
  • Hd
  • Hdh
  • huntingtin
  • HTT
  • htt
  • LOC373520
  • Htt
Epitope
AA 549-679
17
12
11
8
8
7
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Primate, Rat (Rattus)
160
106
65
12
6
2
2
1
1
1
1
Host
Rat
88
81
2
1
Clonality
Monoclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
137
95
76
35
28
26
23
16
13
8
6
4
3
2
Supplier
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Catalog No. ABIN4320705
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4320704 ELISA ICC IF IHC (p) WB Mouse IgG1 Log in to see HU-2E8 0

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 549-679
(17), (12), (11), (8), (8), (7), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Primate, Rat (Rattus)
(160), (106), (65), (12), (6), (2), (2), (1), (1), (1), (1)
Host Rat
(88), (81), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(137), (95), (76), (35), (28), (26), (23), (16), (13), (8), (6), (4), (3), (2)
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling Images
Specificity Huntingtin protein residues 549-679. Huntingtin localization by immunocytochemistry is primarily neuronal. Glia contain very little huntingtin, huntingtin is abundant in perikarya and dendrites of cortical pyramidal cells and in Purkinje cells, and striatal interneurons. Clone mHD549 has been tested in human, rat, and monkey.
Purification Unpurified
Immunogen Huntingtin fusion protein 549-679, fused to Glutathione S-tranferase.
Clone MHD549
Plasmids, Primers & others

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Huntingtin (HTT Antibody Abstract)
Background Gene Symbol: HTT
Gene ID 3064
UniProt P42858
Pathways PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling Images back to top
Application Notes Western Blot 1:100-1:2000, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:500Immunohistochemistry on 3 % /0.1 % glutaraldehyde paraformaldehyde fixed, frozen tissue, using enzymatic detection. See Gutekunst et al, 1998. Does not work on paraffin embedded tissues. For EM, see Gutekunst, 1995 PNAS, 92:8710-8714. Immunocytochemistry/Immunofluorescence. ImmunoBlotting: SDS/PAGE, PVDF membranes, 50 μg/lane total protein, 7.5 % PAGE. Detects 340 kDa ITI5 open reading frame in lymphoblasts from human controls, Additional protein bands are detected in monkey (130-140 kDa). Western Blots {Gutekunst, et al, 1995}. Normal (340 kDa) and mutant ( 340 kDa) bands can be detected in lymphoblast lysates from homozygote or heterozygote HD patients via SDS/4 % PAGE. Abundant in rat tetis (45-50 kDa) (Gutekunst, 1995). Optimal working dilutions and protocols must be determined by end user.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Images back to top
Format Liquid
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.