We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Huntingtin (HTT) Antibodies|
|Epitope||AA 1150-1200 Alternatives|
|Reactivity||Rat (Rattus), Mouse (Murine) Alternatives|
|Conjugate||This Huntingtin antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|2 references available|
Product Details anti-Huntingtin AntibodyTarget Details Huntingtin Application Details Handling References for anti-Huntingtin antibody (ABIN350376) Images
|Specificity||Specific for Huntingtin.|
|Immunogen||A synthetic peptide from aa region 1150-1200 of rat Huntingtin conjugated to an immunogenic carrier protein was used as the antigen. The peptide shares 87% identity with mouse and human sequences.|
|Plasmids, Primers & others|
Target Details HuntingtinProduct Details anti-Huntingtin Antibody Application Details Handling References for anti-Huntingtin antibody (ABIN350376) Images back to top
|Alternative Name||Huntingtin (HTT Antibody Abstract)|
Function: May play a role in microtubule-mediated transport or vesicle function.
Subcellular location: Cytoplasm. Nucleus.
Tissue specificity: Expressed to a high degree in all the regions of the brain of adults and in meiotic cells of the testis. In addition, very low levels are detected in various non-neuronal tissues (heart, muscle, liver, lung and kidney).
Developmental stage: Identified at high levels in neuronal tissues of embryos as early as day 14.5. This expression remains constant in all further development stages (up to the adult). On the other hand the expression in non-neuronal tissues is down-regulated from stage 17.5 day old embryos. Also known as: Huntington disease protein homolog, Htt, HD protein homolog.
|Pathways||PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport|
Application DetailsProduct Details anti-Huntingtin Antibody Target Details Huntingtin Handling References for anti-Huntingtin antibody (ABIN350376) Images back to top
A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Huntingtin Antibody Target Details Huntingtin Application Details References for anti-Huntingtin antibody (ABIN350376) Images back to top
|Reconstitution||Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.|
|Handling Advice||Avoid freeze and thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Expiry Date||12 months|
References for anti-Huntingtin antibody (ABIN350376)Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling Images back to top
Schmitt, Baechner, Megow, Henklein, Hameister, Epplen, Riess: "Expression of the Huntington disease gene in rodents: cloning the rat homologue and evidence for downregulation in non-neuronal tissues during development." in: Human molecular genetics, Vol. 4, Issue 7, pp. 1173-82, 1996
Strong, Tagle, Valdes, Elmer, Boehm, Swaroop, Kaatz, Collins, Albin: "Widespread expression of the human and rat Huntington's disease gene in brain and nonneural tissues." in: Nature genetics, Vol. 5, Issue 3, pp. 259-65, 1994
ImagesProduct Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling References for anti-Huntingtin antibody (ABIN350376) back to top