anti-Rat (Rattus) MTOR antibody for Electron Microscopy

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
pSer2448
65
30
28
23
21
18
18
15
15
12
8
7
7
6
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
408
214
205
16
8
6
6
6
6
5
4
3
1
1
1
Host
Rabbit
355
49
3
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
10
10
9
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Electron Microscopy (EM), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
291
156
123
74
58
52
38
18
16
8
7
7
3
3
2
2
2
1
Supplier
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Catalog No. ABIN153493
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General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope pSer2448
(65), (30), (28), (23), (21), (18), (18), (15), (15), (12), (8), (7), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(408), (214), (205), (16), (8), (6), (6), (6), (6), (5), (4), (3), (1), (1), (1)
Host Rabbit
(355), (49), (3), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(10), (10), (9), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Electron Microscopy (EM), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(291), (156), (123), (74), (58), (52), (38), (18), (16), (8), (7), (7), (3), (3), (2), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN153493) Images
Specificity Reactivity occurs with phosphorylated mTOR from human derived tissues and cells.
Purification Immunogen affinity purified
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2440-2457 of human mTOR.
Isotype IgG
Plasmids, Primers & others

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR antibody (ABIN153493) Images back to top
Antigen
Alternative Name MTOR / FRAP1 (FRAP1 Antibody Abstract)
Background Gene Symbol: MTOR
Gene ID 2475
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR antibody (ABIN153493) Images back to top
Application Notes Western Blot 1:500-1:2000, ELISA 1:100 - 1:2000, Immunohistochemistry 5 μg/mL, Immunohistochemistry-Paraffin 5 μg/mL, Electron Microscopy 1:10000 - 1:100000Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR antibody (ABIN153493) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-MTOR antibody (ABIN153493)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Zhou, Yue, Chan, Tsui, Fung, Sun, Pu, Lau: "Eriocalyxin B, a novel autophagy inducer, exerts anti-tumor activity through the suppression of Akt/mTOR/p70S6K signaling pathway in breast cancer." in: Biochemical pharmacology, Vol. 142, pp. 58-70, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Lisi, Stigliano, Lauriola, Navarra, Dello Russo: "Proinflammatory-activated glioma cells induce a switch in microglial polarization and activation status, from a predominant M2b phenotype to a mixture of M1 and M2a/B polarized cells." in: ASN neuro, Vol. 6, Issue 3, pp. 171-83, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting

Lisi, Laudati, Navarra, Dello Russo: "The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype." in: Journal of neuroinflammation, Vol. 11, pp. 125, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting

Lisi, Navarra, Feinstein, Dello Russo: "The mTOR kinase inhibitor rapamycin decreases iNOS mRNA stability in astrocytes." in: Journal of neuroinflammation, Vol. 8, Issue 1, pp. 1, 2011 (Sample species: Rat (Rattus)). Further details: Western Blotting

Gupta, Dillon, Ziesmer, Feldman, Witzig, Ansell, Cerhan, Novak: "A proliferation-inducing ligand mediates follicular lymphoma B-cell proliferation and cyclin D1 expression through phosphatidylinositol 3-kinase-regulated mammalian target of rapamycin activation." in: Blood, Vol. 113, Issue 21, pp. 5206-16, 2009

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN153493) back to top
Supplier Images
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN153493) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN153493) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (Image 2)
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN153493) Western Blot: TOR/mTOR [p Ser2448] Antibody [ABIN153493] - Detection of a 250 kDa ban...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN153493) Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [ABIN153493] - Used at 5 ug/ml to...