anti-Human NCF1 antibody for ELISA

Recommended NCF1 Antibody (supplied by: Log in to see )

Antigen
Neutrophil Cytosol Factor 1 (NCF1) Antibodies
  • NCF1A
  • NOXO2
  • SH3PXD1A
  • p47phox
  • Ncf-1
  • p47
  • neutrophil cytosolic factor 1
  • NCF1
  • Ncf1
Epitope
C-Term
43
28
16
15
13
11
10
10
8
7
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
236
103
87
14
13
11
11
7
5
1
1
Host
Goat
213
41
1
Clonality
Polyclonal
Conjugate
This NCF1 antibody is un-conjugated
7
7
7
4
4
4
2
2
2
2
2
2
2
2
2
2
2
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
208
139
130
50
33
27
14
12
9
6
2
Options
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Catalog No. ABIN184583
$ 358.02
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531718 IHC ELISA Rabbit IgG AA 301-350, pSer328 Log in to see Polyclonal 2
1 ABIN1531722 IHC ELISA Rabbit IgG AA 341-390, pSer370 Log in to see Polyclonal 2
1 ABIN1532163 IHC ELISA WB Rabbit IgG AA 311-360, pSer345 Log in to see Polyclonal 1
1 ABIN1532746 IF ELISA WB Rabbit IgG AA 281-330 Log in to see Polyclonal 1
1 ABIN1532751 IHC ELISA WB Rabbit IgG AA 341-390 Log in to see Polyclonal 1
1 ABIN1532747 IF IHC ELISA Rabbit IgG AA 301-350 Log in to see Polyclonal 1
1 ABIN1531723 IF IHC ELISA WB Rabbit IgG AA 331-380, pSer359 Log in to see Polyclonal 1
1 ABIN1532752 IHC ELISA WB Rabbit IgG AA 331-380 Log in to see Polyclonal 1
1 ABIN1533187 IHC ELISA WB Rabbit IgG AA 311-360 Log in to see Polyclonal 1
1 ABIN1531717 ELISA WB Rabbit IgG AA 281-330, pSer304 Log in to see Polyclonal 0
1 ABIN337093 ELISA IF IHC IHC (p) WB Goat AA 378-390 Log in to see Polyclonal 0
1 ABIN2878785 ELISA IHC IHC (p) WB Rabbit IgG AA 341-390 Log in to see Polyclonal 0
1 ABIN256632 ELISA IHC (p) WB Goat C-Term Log in to see Polyclonal 1
1 ABIN2889156 ELISA IHC IHC (p) WB Rabbit IgG AA 311-360 Log in to see Polyclonal 0
1 ABIN2888918 ELISA IHC IHC (p) WB Rabbit IgG AA 331-380 Log in to see Polyclonal 0
1 ABIN2888915 IF ELISA WB Rabbit IgG AA 281-330 Log in to see Polyclonal 0
1 ABIN2888916 ELISA IF IHC IHC (p) Rabbit IgG AA 301-350 Log in to see Polyclonal 0
1 ABIN2465255 ELISA WB Goat C-Term Log in to see Polyclonal 0
1 ABIN3186265 ELISA IHC WB Rabbit IgG Tyr905 Log in to see Polyclonal 0
1 ABIN3186264 ELISA IF IHC WB Rabbit IgG Tyr9 Log in to see Polyclonal 0

General

Antigen Neutrophil Cytosol Factor 1 (NCF1) Antibodies
Epitope C-Term
(43), (28), (16), (15), (13), (11), (10), (10), (8), (7), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(236), (103), (87), (14), (13), (11), (11), (7), (5), (1), (1)
Host Goat
(213), (41), (1)
Clonality
Polyclonal
Conjugate This NCF1 antibody is un-conjugated
(7), (7), (7), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Method Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(208), (139), (130), (50), (33), (27), (14), (12), (9), (6), (2)
Pubmed 1 reference available
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Product Details anti-NCF1 Antibody

Target Details NCF1 Application Details Handling References for anti-NCF1 antibody (ABIN184583) Images
Blocking Peptide Blocking peptide for this product available: ABIN369865
Cross-Reactivity Mouse (Murine), Rat (Rattus), Rabbit, Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.
Plasmids, Primers & others

Target Details NCF1

Product Details anti-NCF1 Antibody Application Details Handling References for anti-NCF1 antibody (ABIN184583) Images back to top
Antigen
Alternative Name NCF1 / p47phox (NCF1 Antibody Abstract)
Background Synonyms: NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2
Gene ID 653361, 17969, 114553
NCBI Accession NP_000256
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-NCF1 Antibody Target Details NCF1 Handling References for anti-NCF1 antibody (ABIN184583) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:20000.
Western blot: Approx 46 kDa band observed in lysates of cell line U937 (calculated MW of 44.7 kDa according to NP_000256.3). Recommended concentration: 0.5-1.5 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details References for anti-NCF1 antibody (ABIN184583) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-NCF1 antibody (ABIN184583)

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling Images back to top
Background publications

Davidson-Moncada, Lopez-Lluch, Segal, Dekker: "Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils." in: The Biochemical journal, Vol. 363, Issue Pt 1, pp. 95-103, 2002

Images

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling References for anti-NCF1 antibody (ABIN184583) back to top
Supplier Images
Western Blotting (WB) image for anti-Neutrophil Cytosol Factor 1 (NCF1) (C-Term) antibody (ABIN184583) ABIN184583 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per la...