anti-Pig (Porcine) NCF1 antibody for ELISA

Recommended NCF1 Antibody (supplied by: Log in to see )

Antigen
Neutrophil Cytosol Factor 1 (NCF1) Antibodies
  • NCF1A
  • NOXO2
  • SH3PXD1A
  • p47phox
  • Ncf-1
  • p47
  • neutrophil cytosolic factor 1
  • NCF1
  • Ncf1
Epitope
C-Term
42
29
16
15
14
11
10
10
8
7
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine)
241
103
89
14
13
11
11
7
5
1
1
Host
Goat
217
41
1
Clonality
Polyclonal
Conjugate
This NCF1 antibody is un-conjugated
7
7
7
4
4
4
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
212
143
132
50
35
27
14
12
12
6
2
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Catalog No. ABIN184583
$ 429.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN5872427 ELISA WB Goat Log in to see Polyclonal 0
7 ABIN2564049 ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
4 ABIN1737777 IHC (p) ELISA WB Goat C-Term Log in to see Polyclonal 0
4 ABIN2564336 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN4952024 ELISA IHC (p) WB Goat Ig Fraction Log in to see Polyclonal 0

General

Antigen Neutrophil Cytosol Factor 1 (NCF1) Antibodies
Epitope C-Term
(42), (29), (16), (15), (14), (11), (10), (10), (8), (7), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine)
(241), (103), (89), (14), (13), (11), (11), (7), (5), (1), (1)
Host Goat
(217), (41), (1)
Clonality
Polyclonal
Conjugate This NCF1 antibody is un-conjugated
(7), (7), (7), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(212), (143), (132), (50), (35), (27), (14), (12), (12), (6), (2)
Pubmed 1 reference available
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Product Details anti-NCF1 Antibody

Target Details NCF1 Application Details Handling References for anti-NCF1 antibody (ABIN184583) Images
Purpose NCF1 / p47phox
Blocking Peptide Blocking peptide for this product available: ABIN369865
Sequence SESTKRKLAS AV
Predicted Reactivity Human, Mouse, Rat, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.
Plasmids, Primers & others

Target Details NCF1

Product Details anti-NCF1 Antibody Application Details Handling References for anti-NCF1 antibody (ABIN184583) Images back to top
Antigen
Alternative Name NCF1 (NCF1 Antibody Abstract)
Background NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2
Gene ID 653361
NCBI Accession NP_000256
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-NCF1 Antibody Target Details NCF1 Handling References for anti-NCF1 antibody (ABIN184583) Images back to top
Application Notes

DS WB Results: Approx 48 kDa band observed in lysates of cell line U937 and of Mouse Thymus, and 50 kDa observed in Pig Spleen lysates (calculated MW of 44.7 kDa according to NP_000256.3). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Thymus and Spleen for this particular batch.

DS IHC Results: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:20000.

Restrictions For Research Use only

Handling

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details References for anti-NCF1 antibody (ABIN184583) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-NCF1 antibody (ABIN184583)

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling Images back to top
Background publications

Davidson-Moncada, Lopez-Lluch, Segal, Dekker: "Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils." in: The Biochemical journal, Vol. 363, Issue Pt 1, pp. 95-103, 2002

Images

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling References for anti-NCF1 antibody (ABIN184583) back to top
Supplier Images
Western Blotting (WB) image for anti-Neutrophil Cytosol Factor 1 (NCF1) (C-Term) antibody (ABIN184583) ABIN184583 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per la...