anti-Human PDPK1 antibody for Flow Cytometry

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • ATP5A
  • si:zfos-979f1.3
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3'-phosphoinositide-dependent protein kinase 1
  • 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1
  • ATPDK1
  • AtPDK1
  • T32M21.110
  • T32M21_110
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1 L homeolog
  • 3-phosphoinositide dependent protein kinase-1
  • ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9)
  • 3-phosphoinositide-dependent protein kinase 1
  • pyruvate dehydrogenase kinase, isozyme 1
  • pyruvate dehydrogenase kinase 1
  • Phosphoinositide-dependent kinase 1
  • 3'-phosphoinositide-dependent protein kinase 1
  • PDPK1
  • pdpk1.L
  • pdpk1
  • Pdpk1
  • ATP5G2
  • LOC100380750
  • pdk1
  • PDK1
  • Pdk1
  • pdk-1
Reactivity
Human, Monkey, Rat (Rattus)
444
221
218
18
13
12
11
11
6
5
4
3
2
2
2
1
1
1
1
Host
Mouse
365
72
16
Clonality
Monoclonal
Conjugate
This PDPK1 antibody is un-conjugated
17
16
14
11
11
7
7
7
7
7
7
7
7
7
5
5
4
3
3
3
3
3
3
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
300
148
118
91
66
64
40
31
22
10
6
5
3
2
1
1
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Catalog No. ABIN969347
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.473982 ABIN1108608 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4A11 3
13.473982 ABIN4344574 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4A11 0
13.473982 ABIN1844865 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see 0
13.473982 ABIN5542259 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 7E4G11 0
13.473982 ABIN5542260 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 3H3D9 0
1 ABIN450008 ELISA FACS ICC IF WB Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4880931 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 7E4G11 0
1 ABIN4880932 ELISA FACS ICC WB Mouse IgG1 Log in to see 3H3D9 0
1 ABIN4880420 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 4A11 0
1 ABIN4344590 ELISA FACS ICC IF WB DyLight 755 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344586 ELISA FACS ICC IF WB DyLight 650 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344580 ELISA FACS ICC IF WB Alexa Fluor 405 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344593 ELISA FACS ICC IF WB DyLight 550 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344595 ELISA FACS ICC IF WB DyLight 405 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344581 ELISA FACS ICC IF WB Alexa Fluor 488 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344582 ELISA FACS ICC IF WB Alexa Fluor 647 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344584 FACS APC Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344583 ELISA FACS ICC IF WB Alexa Fluor 700 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344587 ELISA FACS ICC IF FITC Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0
1 ABIN4344588 ELISA FACS ICC IF WB DyLight 680 Mouse IgG1 kappa AA 29-436 Log in to see 2D6 0

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Reactivity Human, Monkey, Rat (Rattus)
(444), (221), (218), (18), (13), (12), (11), (11), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(365), (72), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PDPK1 antibody is un-conjugated
(17), (16), (14), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(300), (148), (118), (91), (66), (64), (40), (31), (22), (10), (6), (5), (3), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 antibody (ABIN969347) Images
Purification Ascites
Immunogen Purified recombinant fragment of human PDK1 expressed in E. Coli.
Clone 4A11
Isotype IgG1

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 antibody (ABIN969347) Images back to top
Antigen
Alternative Name PDK1 (PDPK1 Antibody Abstract)
Background Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. (provided by RefSeq). Tissue specificity: Expressed predominantly in the heart.
Molecular Weight 44 kDa
Gene ID 5163
HGNC 5163
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 antibody (ABIN969347) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 antibody (ABIN969347) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-PDPK1 antibody (ABIN969347)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Product cited in:

Weisberg, Banerji, Wright, Barrett, Ray, Moreno, Catley, Jiang, Hall-Meyers, Sauveur-Michel, Stone, Galinsky, Fox, Kung, Griffin: "Potentiation of antileukemic therapies by the dual PI3K/PDK-1 inhibitor, BAG956: effects on BCR-ABL- and mutant FLT3-expressing cells." in: Blood, Vol. 111, Issue 7, pp. 3723-34, 2008

Pinner, Sahai: "PDK1 regulates cancer cell motility by antagonising inhibition of ROCK1 by RhoE." in: Nature cell biology, Vol. 10, Issue 2, pp. 127-37, 2008

Seong, Jung, Kim, Ha: "3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins." in: The Journal of biological chemistry, Vol. 282, Issue 16, pp. 12272-89, 2007

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 antibody (ABIN969347) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody (ABIN969347) Figure 1: Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody (ABIN969347) Figure 2: Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green) . Bl...
Immunohistochemistry (IHC) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody (ABIN969347) Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody (ABIN969347) Figure 4: Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), J...