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|Antigen||Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies|
|Conjugate||This PIK3CA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-PIK3CA AntibodyTarget Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN360221) Images
|Specificity||This antibody reacts to PK3CA.|
|Purification||Protein A column, followed by peptide affinity purification|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y317 of human PK3CA.|
Target Details PIK3CAProduct Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA antibody (ABIN360221) Images back to top
|Alternative Name||PIK3CA (PIK3CA Antibody Abstract)|
|Background||Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2.Synonyms: 5-bisphosphate 3-kinase catalytic subunit alpha isoform, EC=184.108.40.206, PI3-kinase p110 subunit alpha, PI3K, PIK3CA, Phosphatidylinositol-4, PtdIns-3-kinase p110|
|Gene ID||5290, 9606|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA antibody (ABIN360221) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA antibody (ABIN360221) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PIK3CA antibody (ABIN360221)Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Zhao, Vogt: "Class I PI3K in oncogenic cellular transformation." in: Oncogene, Vol. 27, Issue 41, pp. 5486-96, 2008
Volinia, Hiles, Ormondroyd, Nizetic, Antonacci, Rocchi, Waterfield: "Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene." in: Genomics, Vol. 24, Issue 3, pp. 472-7, 1995
Hiles, Otsu, Volinia, Fry, Gout, Dhand, Panayotou, Ruiz-Larrea, Thompson, Totty: "Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit." in: Cell, Vol. 70, Issue 3, pp. 419-29, 1992
ImagesProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN360221) back to top